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用于临床标本中皮肤癣菌、白色念珠菌和近平滑念珠菌直接检测与诊断的高通量多重聚合酶链反应

High throughput multiplex-PCR for direct detection and diagnosis of dermatophyte species, Candida albicans and Candida parapsilosis in clinical specimen.

作者信息

Vahidnia Ali, Bekers Wouter, Bliekendaal Harry, Spaargaren Joke

机构信息

Molecular Diagnostic Laboratory, Atal-Medial Medical Diagnostic Center, Jan Tooropstraat 138, 1061 AD Amsterdam, The Netherlands; Medical Microbiology Laboratory, Atal-Medial Medical Diagnostic Center, Jan Tooropstraat 138, 1061 AD Amsterdam, The Netherlands.

Molecular Diagnostic Laboratory, Atal-Medial Medical Diagnostic Center, Jan Tooropstraat 138, 1061 AD Amsterdam, The Netherlands; Medical Microbiology Laboratory, Atal-Medial Medical Diagnostic Center, Jan Tooropstraat 138, 1061 AD Amsterdam, The Netherlands.

出版信息

J Microbiol Methods. 2015 Jun;113:38-40. doi: 10.1016/j.mimet.2015.03.024. Epub 2015 Apr 1.

DOI:10.1016/j.mimet.2015.03.024
PMID:25840147
Abstract

We have developed and validated a multiplex-PCR method for detection of dermatophyte spp., Candida albicans and parapsilosis for routine diagnostics. Our m-PCR showed excellent concordance with culture results in 475 clinical samples. Through the rapid diagnosis by our m-PCR, clinicians are able to initiate adequate antimycotic therapy much earlier.

摘要

我们已经开发并验证了一种用于检测皮肤癣菌属、白色念珠菌和近平滑念珠菌的多重聚合酶链反应（m-PCR）方法，用于常规诊断。我们的m-PCR在475份临床样本中与培养结果显示出极好的一致性。通过我们的m-PCR进行快速诊断，临床医生能够更早地开始适当的抗真菌治疗。

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