Li Han, Xu Bing, Wang Danqi, Zhou Yin, Zhang Hongling, Xia Wei, Xu Shunqing, Li Yuanyuan
Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China.
College of Health Science and Nursing, Wuhan Polytechnic University, Wuhan 430023, Hubei, China.
J Biotechnol. 2015 Jun 10;203:97-103. doi: 10.1016/j.jbiotec.2015.03.013. Epub 2015 Apr 1.
In this work, we developed an immunosensor for electrochemical detection of penicillin G at trace level. The biosensor was fabricated by immobilizing anti-penicillin G in a supported bilayer lipid membrane (s-BLM) modified with gold nanoparticles, and the modified electrodes were characterized by the scanning electron microscope (SEM), cyclic voltammetry and electrochemical impedance spectroscopy. The biosensor was able to detect penicillin G with a linear correlation ranging from 3.34×10(-3)ng/L to 3.34×10(3)ng/L and a detection limit of 2.7×10(-4)ng/L, much lower than the maximum residue limit (MRL) of penicillin G in milk (4ppb, equal to 4×10(3)ng/L) set out by the European Union. The mean coefficient variation (CV) of the intra-assays and the inter-assays were 5.4% and 7.7%, respectively. In addition, the concentration of penicillin G in milk samples determined by this biosensor was in good agreement with that determined by high performance liquid chromatography (HPLC) assay.
在本研究中，我们开发了一种用于痕量水平青霉素G电化学检测的免疫传感器。该生物传感器通过将抗青霉素G固定在金纳米粒子修饰的支撑双层脂质膜（s-BLM）中制备而成，并用扫描电子显微镜（SEM）、循环伏安法和电化学阻抗谱对修饰电极进行了表征。该生物传感器能够检测青霉素G，线性相关范围为3.34×10(-3)ng/L至3.34×10(3)ng/L，检测限为2.7×10(-4)ng/L，远低于欧盟规定的牛奶中青霉素G的最大残留限量（MRL）（4ppb，相当于4×10(3)ng/L）。批内和批间的平均变异系数（CV）分别为5.4%和7.7%。此外，该生物传感器测定的牛奶样品中青霉素G的浓度与高效液相色谱（HPLC）测定结果高度一致。
