Soares João Fabio, Carvalho Luis, Maya Leticia, Dutra Fernando, Venzal José Manuel, Labruna Marcelo B
Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando M. de Paiva 87, 05508-900 São Paulo, Brazil.
Laboratorio de Vectores y enfermedades transmitidas and Departamento de Parasitología Veterinaria, Facultad de Veterinaria, Universidad de la República, CENUR Litoral Norte, Rivera 1350, CP 50000 Salto, Uruguay.
Vet Parasitol. 2015 May 30;210(1-2):98-101. doi: 10.1016/j.vetpar.2015.03.013. Epub 2015 Mar 23.
The piroplasm Rangelia vitalii is the etiological agent of canine rangeliosis, a severe disease affecting domestic dogs in South America. Two domestic dogs from two different Departments (Salto and Treinta y Tres) of Uruguay presented with clinical signs such as apathy, anorexia, pale mucous membranes, jaundice, and hemorrhagic manifestations, suggestive of a canine vector-borne disease. Molecular analysis, based on PCR and DNA sequencing of portions of the 18S rRNA gene, revealed that both dogs were infected by R. vitalii. Two consensus sequences, one from Salto and one from Treinta y Tres, differed from each other by only 1 nucleotide (99.8% similarity) and were 99.8-100% identical to corresponding sequences of R. vitalii from Brazil and Argentina available in GenBank. Through phylogenetic analysis inferred by the 18S rRNA gene, the two Uruguayan sequences of R. vitalii were aligned with the corresponding sequences from 7 other R. vitalii sequences available in GenBank (5 from Brazil and, 2 from Argentina) under high bootstrap support. The two dogs of the present study were negative for Ehrlichia canis according to the E. canis-specific real-time PCR assay. Our findings not only confirm the occurrence of R. vitalii in Uruguay but also provide the southernmost record of this re-emerging agent. The only previous report of R. vitalii in Uruguay dated from 1976, a period when molecular analyses were not available. We provide the first molecular detection of R. vitalii in Uruguay. Currently, canine rangeliosis is confirmed to occur in Brazil, Argentina, and Uruguay.
梨形虫类的维氏兰氏梨形虫是犬兰氏梨形虫病的病原体，这是一种影响南美洲家犬的严重疾病。来自乌拉圭两个不同省份（萨尔托省和三十三人省）的两只家犬出现了诸如冷漠、厌食、黏膜苍白、黄疸和出血表现等临床症状，提示为一种犬类媒介传播疾病。基于对18S rRNA基因部分片段的PCR和DNA测序进行的分子分析表明，两只犬均感染了维氏兰氏梨形虫。两个共有序列，一个来自萨尔托省，一个来自三十三人省，彼此仅相差1个核苷酸（相似度为99.8%），并且与GenBank中来自巴西和阿根廷的维氏兰氏梨形虫相应序列的相似度为99.8 - 100%。通过18S rRNA基因推断的系统发育分析，在高自展支持下，将来自乌拉圭的两个维氏兰氏梨形虫序列与GenBank中其他7个维氏兰氏梨形虫序列（5个来自巴西，2个来自阿根廷）的相应序列进行了比对。根据犬埃立希体特异性实时PCR检测，本研究中的两只犬对犬埃立希体呈阴性。我们的研究结果不仅证实了维氏兰氏梨形虫在乌拉圭的存在，还提供了这种再次出现的病原体在最南端的记录。此前关于维氏兰氏梨形虫在乌拉圭的唯一报告可追溯到1976年，当时还无法进行分子分析。我们提供了乌拉圭维氏兰氏梨形虫的首次分子检测。目前，已证实犬兰氏梨形虫病在巴西、阿根廷和乌拉圭均有发生。
