Eiras Diego Fernando, Craviotto María Belén, Baneth Gad, Moré Gastón
Laboratorio DIAP (Diagnóstico en Animales Pequeños), Pueyrredón 1098, B1828ADD Banfield, Buenos Aires, Argentina; Laboratorio de Inmunoparasitología. Departamento de Epizootiología y Salud Pública, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, CC 296, B1900AVW La Plata, Argentina.
Laboratorio DIAP (Diagnóstico en Animales Pequeños), Pueyrredón 1098, B1828ADD Banfield, Buenos Aires, Argentina.
Parasitol Int. 2014 Oct;63(5):729-34. doi: 10.1016/j.parint.2014.06.003. Epub 2014 Jun 23.
A 12-year old mixed breed neutered bitch from Misiones, Argentina, was presented with a history of fever and epistaxis. Blood, bone marrow, and lymph node samples were collected for hematology and cytology. Mild regenerative anemia was recorded and large, round, poorly stained piroplasms (>2.5 μm) were found within erythrocytes in blood and lymph node smears. Nested PCR-RFLP on blood and bone marrow samples was positive for piroplasm DNA. The 18S rRNA gene of piroplasms was targeted. A restriction pattern of a previously unreported piroplasm was observed. The PCR product was sequenced, and the sequence obtained had 99% identity with the Rangelia vitalii sequences from Brazil when compared by BLAST analysis. Further characterization of the detected piroplasm consisted of nearly full-length sequencing (1668 bp) of the 18S rRNA gene of this organism. Those sequences were deposited in GenBank. A phylogenetic analysis indicated that they clustered together with R. vitalii from Brazil but separately from large Babesia species of dogs such as Babesia canis, and from species of Theileria of dogs as well. This is the first report of R. vitalii infection in Argentina, and the first case of canine rangeliosis diagnosed outside Brazil.
一只来自阿根廷米西奥内斯的12岁绝育混血母犬，有发热和鼻出血病史。采集血液、骨髓和淋巴结样本进行血液学和细胞学检查。记录到轻度再生性贫血，在血液和淋巴结涂片的红细胞内发现大型、圆形、染色不佳的梨形虫（>2.5μm）。对血液和骨髓样本进行巢式PCR-RFLP检测，梨形虫DNA呈阳性。靶向梨形虫的18S rRNA基因。观察到一种先前未报道的梨形虫的限制性图谱。对PCR产物进行测序，通过BLAST分析比较，获得的序列与来自巴西的维塔利兰热利虫（Rangelia vitalii）序列有99%的同一性。对检测到的梨形虫的进一步鉴定包括对该生物体18S rRNA基因进行近全长测序（1668 bp）。这些序列已存入GenBank。系统发育分析表明，它们与来自巴西的维塔利兰热利虫聚集在一起，但与犬的大型巴贝斯虫属（如犬巴贝斯虫）以及犬的泰勒虫属物种分开。这是阿根廷首次报道维塔利兰热利虫感染，也是巴西以外首次诊断出犬兰热利病病例。
