Yasuda Michiko Torii, Fujita Kotone, Hosoya Takahiro, Imai Shinjiro, Shimoi Kayoko
Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka , Yada 52-1, Suruga-ku, Shizuoka 422-8526, Japan.
J Agric Food Chem. 2015 Sep 9;63(35):7693-9. doi: 10.1021/acs.jafc.5b00232. Epub 2015 Apr 10.
To elucidate the bioavailability of luteolin and its glycosides in Chrysanthemum morifolium flowers, the absorption and metabolism of luteolin from them was investigated in rats and Caco-2 cells using HPLC and LC-MS. After oral administration of C. morifolium extract (1.7 g/kg body weight (bw), equivalent to 22.8 and 58.3 μmol/kg bw of luteolin and luteolin-7-O-glucoside, respectively) to rats, luteolin and its glycosides were quickly absorbed and luteolin, luteolin monoglucoside, and luteolin monoglucuronide were detected in the plasma. Their levels were highest at 1 h after administration (0.76 ± 0.27 μM). These compounds were also detected in media on the basolateral side from Caco-2 cells treated with the C. morifolium extract. These results suggest that luteolin and luteolin monoglucoside are rapidly absorbed after administration of C. morifolium flower extract and that luteolin, luteolin monoglucoside, and luteolin monoglucuronide may circulate in humans.
为阐明木犀草素及其糖苷在菊花中的生物利用度，采用高效液相色谱法（HPLC）和液相色谱 - 质谱联用技术（LC - MS），在大鼠和人结肠腺癌细胞（Caco - 2细胞）中研究了木犀草素从菊花中的吸收和代谢情况。给大鼠口服菊花提取物（1.7 g/kg体重，分别相当于22.8和58.3 μmol/kg体重的木犀草素和木犀草素 - 7 - O - 葡萄糖苷）后，木犀草素及其糖苷迅速被吸收，血浆中检测到木犀草素、木犀草素单葡萄糖苷和木犀草素单葡萄糖醛酸苷。给药后1小时它们的水平最高（0.76±0.27 μM）。在用菊花提取物处理的Caco - 2细胞的基底外侧培养基中也检测到了这些化合物。这些结果表明，口服菊花提取物后木犀草素和木犀草素单葡萄糖苷迅速被吸收，并且木犀草素、木犀草素单葡萄糖苷和木犀草素单葡萄糖醛酸苷可能在人体内循环。
