McCorriston J
Cytology Department, Royal Infirmary, Glasgow, Scotland.
J Clin Pathol. 1989 Oct;42(10):1101-3. doi: 10.1136/jcp.42.10.1101.
A method of freezing cytological material for long term storage, based on a modification of that used in tissue typing for the storage of lymphocytes, was developed. The method entails the centrifugation of the specimen, adding 1 ml AB or fetal calf serum and 1 ml 20% dimethyl sulphoxide aliquoted into 2 ml tubes, and storage at -70 degrees C. Cytological detail was well preserved in a variety of samples including fine needle aspirates, urine, and bronchial brushings. Three dimensional architecture was also preserved. The method is practical, easy to perform, and allows retrospective studies to be undertaken with the subsequent use of a variety of special staining techniques, including immunocytochemistry.
基于对组织分型中用于储存淋巴细胞的方法进行改进，开发了一种用于长期储存细胞学材料的冷冻方法。该方法包括对标本进行离心，向2ml试管中加入1ml AB血清或胎牛血清以及1ml 20%二甲基亚砜，然后在-70℃下储存。在各种样本中，包括细针穿刺抽吸物、尿液和支气管刷检物，细胞细节都得到了很好的保存。三维结构也得以保留。该方法实用、易于操作，并且允许在后续使用包括免疫细胞化学在内的各种特殊染色技术进行回顾性研究。
