Li Rupeng, Liu Xiping, Sidabras Jason W, Paulson Eric S, Jesmanowicz Andrzej, Nencka Andrew S, Hudetz Anthony G, Hyde James S
Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America.
Department of Dermatology, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America.
PLoS One. 2015 Apr 6;10(4):e0119450. doi: 10.1371/journal.pone.0119450. eCollection 2015.
The aural cavity magnetic susceptibility artifact leads to significant echo planar imaging (EPI) signal dropout in rat deep brain that limits acquisition of functional connectivity fcMRI data. In this study, we provide a method that recovers much of the EPI signal in deep brain. Needle puncture introduction of a liquid-phase fluorocarbon into the middle ear allows acquisition of rat fcMRI data without signal dropout. We demonstrate that with seeds chosen from previously unavailable areas, including the amygdala and the insular cortex, we are able to acquire large scale networks, including the limbic system. This tool allows EPI-based neuroscience and pharmaceutical research in rat brain using fcMRI that was previously not feasible.
耳腔磁化率伪影会导致大鼠深部脑区的回波平面成像（EPI）信号显著丢失，这限制了功能连接性fMRI数据的采集。在本研究中，我们提供了一种方法，可恢复深部脑区的大部分EPI信号。通过中耳穿刺引入液相氟碳化合物，能够在不出现信号丢失的情况下采集大鼠fMRI数据。我们证明，从包括杏仁核和岛叶皮质在内的以前无法获取的区域选择种子点时，能够获取包括边缘系统在内的大规模网络。该工具使基于EPI的大鼠脑区神经科学和药物研究利用fMRI成为可能，而这在以前是不可行的。
