Yoshiyama M, Masada J, Uchida A, Ishida H
Department of Operative Dentistry, Tokushima University School of Dentistry, Japan.
J Dent Res. 1989 Nov;68(11):1498-502. doi: 10.1177/00220345890680110601.
Our aim was to investigate the structural changes of dentinal tubules in specimens obtained from both hypersensitive and naturally desensitized areas in wedge-shaped defects on the same exposed cervical dentin surface of a hypersensitive tooth. A new biopsy technique that makes use of a hollow, cylindrical diamond bur was designed so that specimens from exposed root dentin of vital teeth could be obtained. Twenty-two dentin biopsy pairs were divided into two groups; one was prepared for scanning electron microscopy (SEM) and the other for microradiography (MR). Small hypersensitive areas were identified by a scratch test on exposed human dentin in vivo. SEM observation of the dentin biopsies showed that the orifices of many dentinal tubules in hypersensitive areas were open and that membranous structures appeared on the walls of dentinal tubules. In naturally desensitized areas on the same dentin surface, most of the dentinal tubules were obturated with rhombohedral crystals of all sizes; membranous structures were not observed in these tubules. These results showed that hypersensitivity occurred on the exposed dentin when most of the tubular orifices were open.
我们的目的是研究从同一颗过敏牙暴露的颈部牙本质表面楔形缺损处的过敏区和自然脱敏区获取的标本中牙本质小管的结构变化。设计了一种新的活检技术，该技术使用空心圆柱形金刚石钻针，以便能够从活髓牙暴露的根面牙本质获取标本。22对牙本质活检标本被分为两组；一组准备用于扫描电子显微镜（SEM）观察，另一组用于显微放射照相（MR）。通过在体内对暴露的人牙本质进行划痕试验来确定小的过敏区域。对牙本质活检标本的扫描电子显微镜观察显示，过敏区域许多牙本质小管的管口开放，并且在牙本质小管壁上出现膜状结构。在同一牙本质表面的自然脱敏区域，大多数牙本质小管被各种大小的菱形晶体堵塞；在这些小管中未观察到膜状结构。这些结果表明，当大多数小管管口开放时，暴露的牙本质会出现过敏症状。
