Hayashi Takahito, Ishida Yuko, Kimura Akihiko, Takayasu Tatsunori, Eisenmenger Wolfgang, Kondo Toshikazu
Department of Forensic Medicine, Wakayama Medical University, 811-1 Kmiidera, 641-8509 Wakayama, Japan.
Int J Legal Med. 2004 Dec;118(6):320-5. doi: 10.1007/s00414-004-0468-x. Epub 2004 Aug 3.
An immunohistochemical study combined with morphometry was carried out to examine the time-dependent expression of vascular endothelial growth factor (VEGF) using 53 human skin wounds with different wound ages (groups I: 0-12 h, II: 1-4 days, III: 7-14 days and IV: 17-21 days). In the human wound specimens aged 4-12 h, neutrophils recruited at the wound showed no positive signals for VEGF. With an increase in wound ages of > or =7 days, granulation tissue and angiogenesis were observed, with the migration of macrophages and fibroblasts of which the cytoplasm expressed VEGF-positive reactions. Morphometrically, the average VEGF-positive ratio was highest in group III, followed by that of group IV. In groups III and IV, 13 out of 26 wound samples had VEGF-positive ratios of more than 50%. However, all of the wound samples in groups I and II showed VEGF-positive ratios of less than 50%. With regard to the practical applicability and forensic validity, these observations suggest that a VEGF-positive ratio of more than 50% possibly indicates a wound age of 7 days or more.
采用免疫组织化学结合形态计量学方法，对53例不同伤龄（I组：0 - 12小时，II组：1 - 4天，III组：7 - 14天，IV组：17 - 21天）的人皮肤伤口进行血管内皮生长因子（VEGF）的时间依赖性表达检测。在伤后4 - 12小时的人伤口标本中，伤口处募集的中性粒细胞未显示VEGF阳性信号。随着伤口年龄增加至≥7天，观察到肉芽组织和血管生成，巨噬细胞和成纤维细胞迁移，其细胞质呈现VEGF阳性反应。形态计量学分析显示，III组的平均VEGF阳性率最高，其次是IV组。在III组和IV组中，26个伤口样本中有13个VEGF阳性率超过50%。然而，I组和II组的所有伤口样本VEGF阳性率均低于50%。关于实际应用和法医有效性，这些观察结果表明，VEGF阳性率超过50%可能表明伤口年龄为7天或更长时间。
