Chameettachal Shibu, Murab Sumit, Vaid Radhika, Midha Swati, Ghosh Sourabh
Department of Textile Technology, Indian Institute of Technology, Delhi, India.
J Tissue Eng Regen Med. 2017 Apr;11(4):1212-1229. doi: 10.1002/term.2024. Epub 2015 Apr 7.
Commonly used polymer-based scaffolds often lack visco-elastic properties to serve as a replacement for cartilage tissue. This study explores the effect of reinforcement of silk matrix with chitosan microparticles to create a visco-elastic matrix that could support the redifferentiation of expanded chondrocytes. Goat chondrocytes produced collagen type II and glycosaminoglycan (GAG)-enriched matrix on all the scaffolds (silk:chitosan 1:1, 1:2 and 2:1). The control group of silk-only constructs suffered from leaching out of GAG molecules into the medium. Chitosan-reinforced scaffolds retained a statistically significant (p < 0.02) higher amount of GAG, which in turn significantly increased (p < 0.005) the aggregate modulus (as compared to silk-only controls) of the construct akin to that of native tissue. Furthermore, the microcomposite constructs demonstrated highly pronounced hysteresis at 4% strain up to 400 cycles, mimicking the visco-elastic properties of native cartilage tissue. These results demonstrated a step towards optimizing the design of biomaterial scaffolds used for cartilage tissue engineering. Copyright © 2015 John Wiley & Sons, Ltd.
常用的基于聚合物的支架通常缺乏粘弹性，无法替代软骨组织。本研究探讨了用壳聚糖微粒增强丝基质以创建一种粘弹性基质的效果，该基质可支持扩增软骨细胞的再分化。山羊软骨细胞在所有支架（丝：壳聚糖1:1、1:2和2:1）上产生了富含II型胶原蛋白和糖胺聚糖（GAG）的基质。仅含丝的构建体对照组出现GAG分子渗漏到培养基中的情况。壳聚糖增强的支架保留了统计学上显著更高（p < 0.02）的GAG量，这反过来又显著增加了（p < 0.005）构建体的聚集模量（与仅含丝的对照组相比），类似于天然组织。此外，微复合构建体在4%应变下高达400次循环时表现出高度明显的滞后现象，模拟了天然软骨组织的粘弹性特性。这些结果表明在优化用于软骨组织工程的生物材料支架设计方面迈出了一步。版权所有© 2015约翰威立父子有限公司。
