KAIST Institute for the BioCentury, Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Daejeon, 305-701, South Korea.
School of Materials Science and Engineering, Gwangju Institute of Science and Technology, Gwangju, 500-712, South Korea.
Adv Healthc Mater. 2016 Jan 7;5(1):101-7. doi: 10.1002/adhm.201400834. Epub 2015 Apr 2.
There is considerable interest in developing a common, universal platform for delivering biomacromolecules such as proteins and RNAs into diverse cells with high efficiency. Here, it is shown that carbon nanosyringe arrays (CNSAs) under an applied centrifugal g-force (cf-CNSAs) can deliver diverse bioactive cargos directly into the cytosol of hard-to-transfect cells with relatively high efficiency and reproducibility. The cf-CNSA platform, an optimized version of a previous CNSA-mediated intracellular delivery platform that adds a g-force feature, exhibits more rapid and superior delivery of cargos to various hard-to-transfect cells than is the case in the absence of g-force. Active species, including small interfering RNAs, plasmids, and proteins are successfully transported across plasma membrane barriers into various cells. By overcoming the limitations of currently available transfection methods, the cf-CNSA platform paves the way to universal delivery of a variety of cargos, facilitating the analysis of cellular responses in diverse cell types.
人们对于开发一种通用的、通用的平台来高效地将生物大分子（如蛋白质和 RNA）递送到各种细胞中有着浓厚的兴趣。在这里，研究表明，在施加的离心力（cf-CNSA）下的碳纳米注射器阵列（CNSA）可以将各种生物活性货物直接递送到难以转染的细胞的细胞质中，效率和重现性相对较高。cf-CNSA 平台是以前 CNSA 介导的细胞内递药平台的优化版本，增加了一个离心力功能，与没有离心力的情况相比，它能更快速、更有效地将货物递送到各种难以转染的细胞中。活性物质，包括小干扰 RNA、质粒和蛋白质，成功地穿过质膜屏障进入各种细胞。通过克服现有转染方法的局限性，cf-CNSA 平台为各种货物的通用递送铺平了道路，促进了对不同细胞类型的细胞反应的分析。
