Hara Daiki, Komatsu Hirokazu, Son Aoi, Nishimoto Sei-Ichi, Tanabe Kazuhito
Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Nishikyo-ku, Kyoto, Kyoto 615-8510, Japan.
Bioconjug Chem. 2015 Apr 15;26(4):645-9. doi: 10.1021/acs.bioconjchem.5b00093. Epub 2015 Apr 7.
Dual emission was applied to a molecular probe for the ratiometric sensing of oxygen concentration in a living system. We prepared ruthenium complexes possessing a coumarin unit (Ru-Cou), in which the (3)MLCT phosphorescence of the ruthenium complex was efficiently quenched by molecular oxygen, whereas the coumarin unit emitted constant fluorescence independent of the oxygen concentration. The oxygen status could be determined precisely from the ratio of phosphorescence to fluorescence. We achieved the molecular imaging of cellular oxygen levels using Ru-Cou possessing an alkyl chain, which provided appropriate lipophilicity to increase cellular uptake.
双发射被应用于一种用于活体系统中氧浓度比率传感的分子探针。我们制备了具有香豆素单元的钌配合物（Ru-Cou），其中钌配合物的（3）MLCT磷光被分子氧有效猝灭，而香豆素单元发射与氧浓度无关的恒定荧光。可以根据磷光与荧光的比率精确确定氧状态。我们使用具有烷基链的Ru-Cou实现了细胞氧水平的分子成像，该烷基链提供了适当的亲脂性以增加细胞摄取。
