Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Nishikyo-ku, Kyoto, 615-8510, Japan.
Department of Chemistry and Biological Science, College of Science and Engineering, Aoyama Gakuin University, 5-10-1 Fuchinobe, Chuo-ku, Sagamihara, 252-5258, Japan.
Chembiochem. 2018 May 4;19(9):956-962. doi: 10.1002/cbic.201700685. Epub 2018 Apr 14.
Molecular oxygen in living cells is distributed and consumed inhomogeneously, depending on the activity of each organelle. Therefore, tractable methods that can be used to monitor the oxygen status in each organelle are needed to understand cellular function. Here we report the design of a new oxygen-sensing probe for use in the cell nucleus. We prepared "Ru-Hoechsts", each consisting of a phosphorescent ruthenium complex linked to a Hoechst 33258 moiety, and characterized their properties as oxygen sensors. The Hoechst unit shows strong DNA-binding properties in the nucleus, and the ruthenium complex shows oxygen-dependent phosphorescence. Thus, Ru-Hoechsts accumulated in the cell nucleus and showed oxygen-dependent signals that could be monitored. Of the Ru-Hoechsts prepared in this study, Ru-Hoechst b, in which the ruthenium complex and the Hoechst unit were linked through a hexyl chain, showed the most suitable properties for monitoring the oxygen status. Ru-Hoechsts are probes with high potential for visualizing oxygen fluctuations in the nucleus.
在活细胞中，分子氧的分布和消耗是不均匀的，这取决于每个细胞器的活性。因此，需要开发一种可行的方法来监测每个细胞器中的氧状态，以了解细胞功能。在这里，我们报告了一种用于细胞核氧传感探针的设计。我们制备了“Ru-Hoechsts”，每个探针由一个连接到 Hoechst 33258 部分的磷光钌配合物组成，并对其作为氧传感器的性质进行了表征。Hoechst 单元在核内具有很强的 DNA 结合特性，而钌配合物则表现出氧依赖性磷光。因此，Ru-Hoechsts 在细胞核内积累，并表现出可监测的氧依赖性信号。在本研究中制备的 Ru-Hoechsts 中，通过己基链连接钌配合物和 Hoechst 单元的 Ru-Hoechst b 表现出最适合监测氧状态的性质。Ru-Hoechsts 是一种具有很大潜力的探针，可用于可视化细胞核内氧波动。
