Department of Chemistry and Biological Science, College of Science and Engineering, Aoyama Gakuin University, 5-10-1 Fuchinobe, Chuo-ku, Sagamihara 252-5258, Japan.
Department of Chemistry and Biological Science, College of Science and Engineering, Aoyama Gakuin University, 5-10-1 Fuchinobe, Chuo-ku, Sagamihara 252-5258, Japan.
Bioorg Med Chem. 2018 Sep 1;26(16):4595-4601. doi: 10.1016/j.bmc.2018.03.043. Epub 2018 Mar 30.
Ruthenium complexes are very useful phosphorescent probes for the visualization of hypoxia. We designed and synthesized three ruthenium complexes possessing bromopyrene, naphthalene, or anthracene units to improve the oxygen response. These ruthenium complexes provided strong phosphorescence under hypoxic conditions, while an increase in oxygen concentration led to a decrease in phosphorescence intensity. Among the ruthenium complexes, that with a bromopyrene unit (Ru-BrPy) had the best properties. This showed good cellular uptake and bright emission in cells, and had the highest sensitivity for molecular oxygen. Thus, Ru-BrPy is a promising candidate as a molecular probe for detecting cellular hypoxia.
钌配合物是用于缺氧可视化的非常有用的磷光探针。我们设计并合成了三种具有溴代苝、萘或蒽单元的钌配合物，以提高对氧的响应。这些钌配合物在缺氧条件下提供强磷光，而氧浓度的增加导致磷光强度降低。在这些钌配合物中，带有溴代苝单元的配合物（Ru-BrPy）具有最佳性质。它在细胞中表现出良好的细胞摄取和明亮的发射，并对分子氧具有最高的灵敏度。因此，Ru-BrPy 是一种很有前途的用于检测细胞缺氧的分子探针候选物。
