Kusubata Masashi, Koyama Yoh-Ichi, Tometsuka Chisa, Shigemura Yasutaka, Sato Kenji
a Research Institute of Biomatrix , Nippi Inc. , Toride , Japan.
Biosci Biotechnol Biochem. 2015;79(8):1356-61. doi: 10.1080/09168451.2015.1027653. Epub 2015 Apr 7.
Generation of collagen dipeptides and deposition of orally administered prolylhydroxyproline (Pro-Hyp) in local inflammatory sites were examined in mice with hapten (2,4-dinitrofluorobenzene)-induced dermatitis in the ear. Pro-Hyp content in the hapten-treated ear was significantly higher in the chronic phase of contact dermatitis than the vehicle control. In contrast, hydroxyprolylglycine contents remained at lower levels in all cases compared to Pro-Hyp. Four hours after the ingestion of [(13)C5,(15)N]Pro and [(13)C5,(15)N]Pro-Hyp, labeled-Pro-Hyp and Pro, respectively, appeared only in the ear with dermatitis. Thus, Pro-Hyp is generated and degraded as part of the rapid synthesis and degradation of collagen in the ear with dermatitis. In addition to the endogenously generated Pro-Hyp, the orally administered Pro-Hyp was deposited in the ears.
在通过半抗原（2,4-二硝基氟苯）诱导耳部发生皮炎的小鼠中，研究了胶原二肽的生成以及口服给予的脯氨酰羟脯氨酸（Pro-Hyp）在局部炎症部位的沉积情况。在接触性皮炎的慢性期，经半抗原处理的耳部中的Pro-Hyp含量显著高于赋形剂对照组。相比之下，与Pro-Hyp相比，羟脯氨酰甘氨酸含量在所有情况下均保持在较低水平。摄入[(13)C5,(15)N]Pro和[(13)C5,(15)N]Pro-Hyp 4小时后，标记的Pro-Hyp和Pro分别仅出现在患有皮炎的耳部。因此，Pro-Hyp作为皮炎耳部中胶原蛋白快速合成和降解的一部分而产生和降解。除了内源性生成的Pro-Hyp外，口服给予的Pro-Hyp也沉积在耳部。
