Roy Choudhury Gourav, Winters Ali, Rich Ryan M, Ryou Myoung-Gwi, Gryczynski Zygmunt, Yuan Fang, Yang Shao-Hua, Liu Ran
Department of Pharmacology and Neuroscience, Institute for Aging and Alzheimer's Disease Research, University of North Texas Health Science Center, Fort Worth, Texas, United States of America.
Department of Cell Biology & Immunology, University of North Texas Health Science Center, Fort Worth, Texas, United States of America.
PLoS One. 2015 Apr 7;10(4):e0123096. doi: 10.1371/journal.pone.0123096. eCollection 2015.
Astrocytes outnumber neurons and serve many metabolic and trophic functions in the mammalian brain. Preserving astrocytes is critical for normal brain function as well as for protecting the brain against various insults. Our previous studies have indicated that methylene blue (MB) functions as an alternative electron carrier and enhances brain metabolism. In addition, MB has been shown to be protective against neurodegeneration and brain injury. In the current study, we investigated the protective role of MB in astrocytes. Cell viability assays showed that MB treatment significantly protected primary astrocytes from oxygen-glucose deprivation (OGD) & reoxygenation induced cell death. We also studied the effect of MB on cellular oxygen and glucose metabolism in primary astrocytes following OGD-reoxygenation injury. MB treatment significantly increased cellular oxygen consumption, glucose uptake and ATP production in primary astrocytes. In conclusion our study demonstrated that MB protects astrocytes against OGD-reoxygenation injury by improving astrocyte cellular respiration.
在哺乳动物大脑中，星形胶质细胞的数量超过神经元，并发挥着许多代谢和营养功能。保护星形胶质细胞对于正常脑功能以及保护大脑免受各种损伤至关重要。我们之前的研究表明，亚甲蓝（MB）作为一种替代电子载体发挥作用，并增强脑代谢。此外，MB已被证明对神经退行性变和脑损伤具有保护作用。在本研究中，我们研究了MB在星形胶质细胞中的保护作用。细胞活力测定表明，MB处理可显著保护原代星形胶质细胞免受氧糖剥夺（OGD）和复氧诱导的细胞死亡。我们还研究了MB对OGD-复氧损伤后原代星形胶质细胞中细胞氧和葡萄糖代谢的影响。MB处理显著增加了原代星形胶质细胞的细胞耗氧量、葡萄糖摄取和ATP生成。总之，我们的研究表明，MB通过改善星形胶质细胞的细胞呼吸来保护星形胶质细胞免受OGD-复氧损伤。
