Avila-Rincon M J, Ferrero M L, Alonso E, García-Luque I, Díaz-Ruíz J R
U.E.I. Fitopatología, Centro de Investigaciones Biológicas, CSIC, Madrid, Spain.
J Gen Virol. 1989 Nov;70 ( Pt 11):3025-31. doi: 10.1099/0022-1317-70-11-3025.
The nucleotide sequences of the 5' and 3' non-coding regions of pepper mild mottle virus strain S (PMMV-S) RNA were determined; they are more like corresponding sequences of tomato mosaic virus (ToMV) RNA than those of any other tobamovirus reported so far. The 5' leader contains a 68 nucleotide guanosine-free sequence which differs in several nucleotides from the corresponding sequences in genomic RNA of tobacco mosaic virus (TMV) and ToMV. The messenger activity of PMMV-S RNA in vitro and the polypeptide translation products made were similar to those of TMV RNA. It therefore seems unlikely that qualitative or quantitative differences in translation in vivo account for the milder symptoms induced by PMMV-S, and its lesser replication, than TMV. The 3' non-coding region of PMMV-S RNA is 199 nucleotides long and can be folded into the same secondary structure as the RNA of other tobamoviruses.
测定了辣椒轻斑驳病毒S株（PMMV-S）RNA 5'和3'非编码区的核苷酸序列；与迄今报道的任何其他烟草花叶病毒相比，它们更类似于番茄花叶病毒（ToMV）RNA的相应序列。5'前导序列包含一个68个核苷酸的无鸟苷序列，该序列在几个核苷酸上与烟草花叶病毒（TMV）和ToMV基因组RNA中的相应序列不同。PMMV-S RNA在体外的信使活性以及产生的多肽翻译产物与TMV RNA相似。因此，体内翻译的定性或定量差异似乎不太可能解释PMMV-S诱导的症状比TMV轻以及其复制较少的原因。PMMV-S RNA的3'非编码区长199个核苷酸，可以折叠成与其他烟草花叶病毒RNA相同的二级结构。
