Riviere C J, Pot J, Tremaine J H, Rochon D M
Research Station, Agriculture Canada, Vancouver, British Columbia.
J Gen Virol. 1989 Nov;70 ( Pt 11):3033-42. doi: 10.1099/0022-1317-70-11-3033.
Complementary DNA copies of the genomic RNA of melon necrotic spot virus (MNSV) have been cloned and the region deduced to encode the coat protein has been sequenced. The putative coat protein coding region, located near the 3' end of the genome, consists of 1170 nucleotides and has the potential to encode a 390 amino acid protein of Mr 41,840. Our data show that although MNSV is a carmovirus, its coat protein more closely resembles those of the tombusviruses than those of the carmoviruses sequenced to date, in both the extent of sequence similarity and in the length of the random/arm and protruding domains of the coat protein. Furthermore, dot matrix comparisons revealed sequence similarity between the coat protein protruding domains of MNSV and the cucumber necrosis tombusvirus. This similarity may be involved in one or more of the biological properties these two viruses share, such as the ability to infect cucumbers naturally and to be transmitted by the soil-inhabiting fungus Olpidium radicale.
甜瓜坏死斑点病毒（MNSV）基因组RNA的互补DNA拷贝已被克隆，并且已对推测编码外壳蛋白的区域进行了测序。推测的外壳蛋白编码区位于基因组3'端附近，由1170个核苷酸组成，有可能编码一个390个氨基酸、分子量为41,840的蛋白质。我们的数据表明，尽管MNSV是一种香石竹潜隐病毒属病毒，但其外壳蛋白在序列相似程度以及外壳蛋白随机/臂和突出结构域的长度方面，与已测序的香石竹潜隐病毒属病毒相比，更类似于番茄丛矮病毒属病毒。此外，点阵比较揭示了MNSV外壳蛋白突出结构域与黄瓜坏死番茄丛矮病毒之间的序列相似性。这种相似性可能与这两种病毒共有的一种或多种生物学特性有关，例如自然感染黄瓜以及由土壤真菌油壶菌传播的能力。
