State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, People's Republic of China.
ACS Appl Mater Interfaces. 2015 May 6;7(17):8990-8. doi: 10.1021/acsami.5b01552. Epub 2015 Apr 22.
In this work, a facile colorimetric method is developed for quantitative detection of human serum albumin (HSA) based on the antiaggregation effect of gold nanoparticles (Au NPs) in the presence of HSA. The citrate-capped Au NPs undergo a color change from red to blue when melamine is added as a cross-linker to induce the aggregation of the NPs. Such an aggregation is efficiently suppressed upon the adsorption of HSA on the particle surface. This method provides the advantages of simplicity and cost-efficiency for quantitative detection of HSA with a detection limit of ∼1.4 nM by monitoring the colorimetric changes of the Au NPs with UV-vis spectroscopy. In addition, this approach shows good selectivity for HSA over various amino acids, peptides, and proteins and is qualified for detection of HSA in a biological sample. Such an antiaggregation effect can be further extended to fabricate an INHIBIT logic gate by using HSA and melamine as inputs and the color changes of Au NPs as outputs, which may have application potentials in point-of-care medical diagnosis.
在这项工作中，开发了一种基于金纳米粒子（Au NPs）在存在人血清白蛋白（HSA）时的抗聚集效应的简便比色法，用于定量检测 HSA。当三聚氰胺作为交联剂添加时，柠檬酸钠封端的 Au NPs 会从红色变为蓝色，从而诱导 NPs 聚集。当 HSA 吸附在粒子表面上时，这种聚集会被有效地抑制。该方法通过监测 Au NPs 的比色变化，利用紫外-可见光谱法提供了一种简单、经济的定量检测 HSA 的方法，检测限约为 1.4 nM。此外，该方法对 HSA 具有良好的选择性，优于各种氨基酸、肽和蛋白质，可用于检测生物样品中的 HSA。这种抗聚集效应可以进一步扩展，通过使用 HSA 和三聚氰胺作为输入，Au NPs 的颜色变化作为输出，构建 INHIBIT 逻辑门，这可能在即时医疗诊断中有应用潜力。
