Deng Hao-Hua, Shi Xiao-Qiong, Balasubramanian Paramasivam, Huang Kai-Yuan, Xu Ying-Ying, Huang Zhong-Nan, Peng Hua-Ping, Chen Wei
Fujian Key Laboratory of Drug Target Discovery and Structural and Functional Research, School of Pharmacy, Fujian Medical University, Fuzhou 350004, China.
Nanomaterials (Basel). 2020 Feb 7;10(2):281. doi: 10.3390/nano10020281.
This study puts forward an efficient method for protein detection in virtue of the tremendous fluorescence enhancement property of 6-aza-2-thio-thymine protected gold nanoclusters (ATT-AuNCs). In-depth studies of the protein-induced photoluminescence enhancement mechanism illustrate the mechanism of the interaction between ATT-AuNCs and protein. This new-established probe enables feasible and sensitive quantification of the concentrations of total protein in real samples, such as human serum, human plasma, milk, and cell extracts. The results of this proposed method are in good agreement with those determined by the classical bicinchoninic acid method (BCA method).
本研究借助6-氮杂-2-硫代胸腺嘧啶保护的金纳米团簇（ATT-AuNCs）的巨大荧光增强特性，提出了一种高效的蛋白质检测方法。对蛋白质诱导的光致发光增强机制的深入研究阐明了ATT-AuNCs与蛋白质之间的相互作用机制。这种新建立的探针能够对实际样品（如人血清、人血浆、牛奶和细胞提取物）中的总蛋白浓度进行可行且灵敏的定量分析。该方法的结果与经典的二辛可宁酸法（BCA法）测定的结果高度一致。
