Kaya Murat, Bağrıaçık Nil, Seyyar Osman, Baran Talat
Department of Biotechnology and Molecular Biology, Faculty of Arts and Sciences, Aksaray University, Aksaray, Turkey.
Science and Technology Application and Research Center, Aksaray University, Aksaray, Turkey.
Arch Insect Biochem Physiol. 2015 Aug;89(4):204-17. doi: 10.1002/arch.21237. Epub 2015 Apr 7.
There has been no study on the chitin structure of wasp species. Here, we selected the three most common wasp species belonging to the family Vespidae for chitin extraction and characterization. Chitin was isolated from each wasp species and characterized by Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffractometry (XRD), elemental analysis (EA), and scanning electron microscopy (SEM). The chitin contents of Vespa crabro, Vespa orientalis, and Vespula germanica were 8.3, 6.4, and 11.9%, respectively. The crystalline index (CrI) values for the chitin extracted from each species were 69.88, 53.92, and 50%, respectively. The most important finding of the study is that although the same method was used to extract chitin from each of the three wasp species, the degree of acetylation was different: for V. crabro and V. orientalis it was 96.85 and 99.82% (the chitin was extremely pure), respectively, whereas that for V. germanica the chitin was 79.83%.
目前尚未有关于黄蜂物种几丁质结构的研究。在此,我们选取了胡蜂科三种最常见的黄蜂物种进行几丁质提取和表征。从每种黄蜂物种中分离出几丁质,并通过傅里叶变换红外光谱(FTIR)、热重分析(TGA)、X射线衍射(XRD)、元素分析(EA)和扫描电子显微镜(SEM)对其进行表征。大胡蜂、东方胡蜂和德国黄胡蜂的几丁质含量分别为8.3%、6.4%和11.9%。从每种物种中提取的几丁质的结晶度指数(CrI)值分别为69.88%、53.92%和50%。该研究最重要的发现是,尽管使用相同的方法从三种黄蜂物种中提取几丁质,但乙酰化程度不同:大胡蜂和东方胡蜂的乙酰化程度分别为96.85%和99.82%(几丁质极其纯净),而德国黄胡蜂的几丁质乙酰化程度为79.83%。