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从亚洲大黄蜂(Lepeletier 1836)(膜翅目:胡蜂科)中提取和理化特性分析甲壳素。

Extraction and Physicochemical Characterization of Chitin Derived from the Asian Hornet, Lepeletier 1836 (Hym.: Vespidae).

机构信息

Academy of Veterinary Sciences of Galicia, Edificio EGAP, Rúa Madrid, No. 2-4, 15707 Santiago de Compostela, (A Coruña), Spain.

Departamento de Química Orgánica, Facultad de Ciencias, Universidad of Santiago De Compostela, Alfonso X el Sabio, 27002 Lugo, Spain.

出版信息

Molecules. 2020 Jan 17;25(2):384. doi: 10.3390/molecules25020384.

Abstract

Fifteen years ago, at least one multimated female yellow-legged Asian hornet ( Lepeletier 1836) arrived in France, which gave rise to a pan-European invasion. In this study, the isolation and characterization of chitin (CHI) that was obtained from (CHI) is described. In addition, an easy procedure is carried out to capture the raw insect, selectively and with high rates of success. The chitin contents of dry VV was observed to be 11.7%. Fourier transform infrared spectroscopy (FTIR), solid-state NMR (ssNMR), elemental analysis (EA), scanning electron microscopy (SEM), and thermogravimetric analysis (TG) characterized the physicochemical properties of CHI. The obtained CHI is close to pure (43.47% C, 6.94% H, and 6.85% N), and full acetylated with a value of 95.44%. Additionally, lifetime and kinetic parameters such as activation E and the frequency factor A using model-free and model-fitting methods, were determined. For CHI the solid state mechanism that follows the thermodegradation is of type F2 (random nucleation around two nuclei). The invasive Asian hornet is a promising alternative source of CHI, based on certain factors, such as the current and probable continued abundance of the quantity and quality of the product obtained.

摘要

十五年前,至少有一种多态性雌性黄脚亚洲大黄蜂(Lepeletier 1836)抵达法国,引发了泛欧入侵。本研究描述了从(亚洲大黄蜂)中分离和表征几丁质(CHI)的过程。此外,还采用了一种简单的程序来捕获原始昆虫,具有较高的选择性和成功率。干 VV 的几丁质含量观察到为 11.7%。傅里叶变换红外光谱(FTIR)、固态 NMR(ssNMR)、元素分析(EA)、扫描电子显微镜(SEM)和热重分析(TG)对 CHI 的物理化学性质进行了表征。得到的 CHI 接近纯(43.47% C、6.94% H 和 6.85% N),完全乙酰化,值为 95.44%。此外,还使用无模型和模型拟合方法确定了寿命和动力学参数,如活化 E 和频率因子 A。对于 CHI,遵循热降解的固态机制是类型 F2(在两个核周围随机成核)。入侵的亚洲大黄蜂是 CHI 的有前途的替代来源,这基于某些因素,例如当前和可能持续的数量和质量的丰富性和产品的质量。

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