Terao Naoko, Takamatsu Shinji, Minehira Tomomi, Sobajima Tomoaki, Nakayama Kotarosumitomo, Kamada Yoshihiro, Miyoshi Eiji
Naoko Terao, Shinji Takamatsu, Tomomi Minehira, Tomoaki Sobajima, Kotarosumitomo Nakayama, Yoshihiro Kamada, Eiji Miyoshi, Department of Molecular Biochemistry and Clinical Investigation, Osaka University Graduate School of Medicine, Suita 565-0871, Japan.
World J Gastroenterol. 2015 Apr 7;21(13):3876-87. doi: 10.3748/wjg.v21.i13.3876.
To evaluate/isolate cancer stem cells (CSCs) from tissue or cell lines according to various definitions and cell surface markers.
Lectin microarray analysis was conducted on CSC-like fractions of the human pancreatic cancer cell line Panc1 by establishing anti-cancer drug-resistant cells. Changes in glycan structure of CSC-like cells were also investigated in sphere-forming cells as well as in CSC fractions obtained from overexpression of CD24 and CD44.
Several types of fucosylation were increased under these conditions, and the expression of fucosylation regulatory genes such as fucosyltransferases, GDP-fucose synthetic enzymes, and GDP-fucose transporters were dramatically enhanced in CSC-like cells. These changes were significant in gemcitabine-resistant cells and sphere cells of a human pancreatic cancer cell line, Panc1. However, downregulation of cellular fucosylation by knockdown of the GDP-fucose transporter did not alter gemcitabine resistance, indicating that increased cellular fucosylation is a result of CSC-like transformation.
Fucosylation might be a biomarker of CSC-like cells in pancreatic cancer.
根据各种定义和细胞表面标志物，从组织或细胞系中评估/分离癌症干细胞（CSCs）。
通过建立抗癌药物耐药细胞，对人胰腺癌细胞系Panc1的CSC样组分进行凝集素微阵列分析。还在成球细胞以及从CD24和CD44过表达获得的CSC组分中研究了CSC样细胞聚糖结构的变化。
在这些条件下，几种岩藻糖基化类型增加，并且岩藻糖基化调节基因如岩藻糖基转移酶、GDP-岩藻糖合成酶和GDP-岩藻糖转运蛋白的表达在CSC样细胞中显著增强。这些变化在人胰腺癌细胞系Panc1的吉西他滨耐药细胞和成球细胞中很明显。然而，通过敲低GDP-岩藻糖转运蛋白下调细胞岩藻糖基化并没有改变吉西他滨耐药性，表明细胞岩藻糖基化增加是CSC样转化的结果。
岩藻糖基化可能是胰腺癌中CSC样细胞的生物标志物。
