Mirpour Mirsasan, Gholizadeh Siahmazgi Zohreh, Sharifi Kiasaraie Masoumeh
Microbiology Department, Islamic Azad University Lahijan Branch, Iran.
Chemistry Department, Islamic Azad University Rasht Branch, Iran.
J Oral Biol Craniofac Res. 2015 Jan-Apr;5(1):7-10. doi: 10.1016/j.jobcr.2015.02.002. Epub 2015 Feb 25.
Antimicrobial compounds from herbal sources have good therapeutic potential. In this study, the antibacterial effects of clove and gall nut, methanolic and ethanolic extractions, were evaluated for their effect on Streptococcus mutans PTCC 1683 and Streptococcus salivarius PTCC 1448, as both the two cause oral diseases.
The clove and gall nut methanolic and ethanolic extracts were prepared and antibacterial activity was evaluated for S. mutans and S. salivarius in the base of inhibition zone diameter using agar diffusion method. In this part minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were assessed.
These extracts showed effective antibacterial activity on bacteria. Antibacterial activity of Methanolic extract of clove was more than that of ethanolic extract, and ethanolic extracts of gall nut had antibacterial activity more than that of methanolic extracts. MIC and MBC results for clove methanolic extract were 1.5 mg/ml and 3 mg/ml for S. mutans and 6.25 mg/ml and 12.5 mg/ml for S. salivarius, respectively. These results for clove ethanolic extracts were 12.5 mg/ml and 25 mg/ml for S. mutans and 25 mg/ml and 50 mg/ml for S. salivarius, respectively. MIC and MBC results for gall nut methanolic extract were 25 mg/ml and 50 mg/ml for S. mutans and 12.5 mg/ml and 25 mg/ml for S. salivarius, respectively. These results for gall nut ethanolic extracts were 3.1 mg/ml and 6.2 mg/ml for S. mutans and 25 mg/ml and 50 mg/ml for S. salivarius, respectively.
The results showed effective antibacterial activity using clove and gall nut methanolic extracts. If other properties such as tolerance of tissue can also be studied, these extracts can be used as a mouthwash.
来自草药来源的抗菌化合物具有良好的治疗潜力。在本研究中，评估了丁香和五倍子的甲醇提取物和乙醇提取物对变形链球菌PTCC 1683和唾液链球菌PTCC 1448的抗菌作用，因为这两种细菌都会引发口腔疾病。
制备丁香和五倍子的甲醇提取物和乙醇提取物，并采用琼脂扩散法，根据抑菌圈直径评估其对变形链球菌和唾液链球菌的抗菌活性。在这部分实验中，还评估了最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。
这些提取物对细菌显示出有效的抗菌活性。丁香甲醇提取物的抗菌活性高于乙醇提取物，五倍子乙醇提取物的抗菌活性高于甲醇提取物。丁香甲醇提取物对变形链球菌的MIC和MBC结果分别为1.5毫克/毫升和3毫克/毫升，对唾液链球菌分别为6.25毫克/毫升和12.5毫克/毫升。丁香乙醇提取物对变形链球菌的这些结果分别为12.5毫克/毫升和25毫克/毫升，对唾液链球菌分别为25毫克/毫升和50毫克/毫升。五倍子甲醇提取物对变形链球菌的MIC和MBC结果分别为25毫克/毫升和50毫克/毫升，对唾液链球菌分别为12.5毫克/毫升和25毫克/毫升。五倍子乙醇提取物对变形链球菌的这些结果分别为3.1毫克/毫升和6.2毫克/毫升，对唾液链球菌分别为25毫克/毫升和50毫克/毫升。
结果表明丁香和五倍子的甲醇提取物具有有效的抗菌活性。如果还能研究其他特性，如组织耐受性，这些提取物可作为漱口水使用。
