Panijpan Bhinyo, Laosinchai Parames, Senapin Saengchan, Kowasupat Chanon, Ruenwongsa Pintip, Kühne Jens, Phiwsaiya Kornsunee
Multidisciplinary Unit, Faculty of Science, Mahidol University, Phayathai, Bangkok, Thailand.
Institute for Innovative Learning, Mahidol University, Salaya, Nakhon Pathom, Thailand.
Meta Gene. 2015 Mar 12;4:17-28. doi: 10.1016/j.mgene.2015.02.003. eCollection 2015 Jun.
Air-breathing fish species of the genus Trichopsis have been reported in Cambodia, Lao PDR, Indonesia, Malaysia, Singapore, Thailand and Vietnam. It is only in Thailand that all three recognized species (Trichopsis vittata, Trichopsis schalleri and Trichopsis pumila), as judged by distinct external features, are found. Cambodia and Lao PDR harbor two species each. The present work involves first-time DNA sequencing and analysis based on mitochondrial (COI) and nuclear (RAG1) DNA of numerous specimens of these species and specimens of a controversial Phetchaburi (Thailand) fish population with a mixed outward appearance. In addition to confirming the morphologically clear-cut taxonomic division of the three fish species, our DNA results show that whereas the T. pumila populations form one single species, there are cryptic species in the T. vittata and T. schalleri populations and possibly a new one in the latter. Members of the putative Phetchaburi fish population have been proven to be hybrids between T. pumila and T. vittata. In addition, a new the phylogenetic tree indicating ancestral relationships is also presented. This study should generate further research to find new/cryptic species of the genus Trichopsis in all countries harboring the fish.
柬埔寨、老挝、印度尼西亚、马来西亚、新加坡、泰国和越南均有呼吸空气的斗鱼属鱼类的报道。只有在泰国能发现所有三种公认的斗鱼（珍珠斗鱼、施氏斗鱼和侏儒斗鱼），这是根据明显的外部特征判断的。柬埔寨和老挝各有两种斗鱼。本研究首次对这些斗鱼物种的大量样本以及泰国碧武里一个外观混杂且存在争议的斗鱼种群样本进行了基于线粒体（COI）和核基因（RAG1）DNA的测序和分析。除了确认这三种斗鱼在形态学上明确的分类划分外，我们的DNA分析结果还表明，侏儒斗鱼种群构成单一物种，而珍珠斗鱼和施氏斗鱼种群中存在隐存种，后者可能还有一个新物种。碧武里斗鱼种群的成员已被证明是侏儒斗鱼和珍珠斗鱼的杂交种。此外，还呈现了一个表明祖先关系的新系统发育树。本研究应推动进一步研究，以便在所有有斗鱼的国家找到斗鱼属的新物种/隐存种。
