Mignon Charlotte, Sodoyer Régis, Werle Bettina
Technology Research Institute Bioaster, 317 avenue Jean-Jaurés, 69007 Lyon, France.
Pathogens. 2015 Apr 3;4(2):157-81. doi: 10.3390/pathogens4020157.
The continuously improving sophistication of molecular engineering techniques gives access to novel classes of bio-therapeutics and new challenges for their production in full respect of the strengthening regulations. Among these biologic agents are DNA based vaccines or gene therapy products and to a lesser extent genetically engineered live vaccines or delivery vehicles. The use of antibiotic-based selection, frequently associated with genetic manipulation of microorganism is currently undergoing a profound metamorphosis with the implementation and diversification of alternative selection means. This short review will present examples of alternatives to antibiotic selection and their context of application to highlight their ineluctable invasion of the bio-therapeutic world.
分子工程技术的日益复杂，使得新型生物治疗药物不断涌现，同时也给在严格遵守日益强化的法规的前提下生产这些药物带来了新挑战。这些生物制剂包括基于DNA的疫苗或基因治疗产品，以及在较小程度上的基因工程活疫苗或递送载体。基于抗生素的筛选方法常与微生物的基因操作相关联，随着替代筛选方法的实施和多样化，目前这种方法正在经历深刻的变革。这篇简短的综述将介绍抗生素筛选的替代方法实例及其应用背景，以突出它们在生物治疗领域不可避免的侵入。
