诱导蛋白-10 和肝素结合血凝素能否提高低结核发病率国家中结核分枝杆菌感染患者的检出率？

Could inducible protein-10 and heparin-binding hemagglutinin improve the detection of Mycobacterium tuberculosis-infected subjects in a country with low incidence of tuberculosis?

机构信息

From the 1 Department of Biomedical Sciences.

出版信息

Infect Dis (Lond). 2015 Aug;47(8):563-7. doi: 10.3109/23744235.2015.1031173. Epub 2015 Apr 9.

DOI:10.3109/23744235.2015.1031173

PMID:25856090

Abstract

BACKGROUND

This study aimed to evaluate inducible protein-10 (IP-10) as a biomarker besides interferon-gamma (IFN-γ) to improve the identification of active tuberculosis (TB) and latent tubercular infection (LTBI) in a country with a low incidence of TB.

METHODS

Whole blood from Mycobacterium tuberculosis-infected subjects was stimulated with region-of-difference-1 (RD1)-specific peptides and with heparin-binding hemagglutinin (HBHA) to determine the release of IP-10 and IFN-γ.

RESULTS

No statistically significant difference was observed between positive rates of IP-10 and IFN-γ after RD1-specific peptide stimulation in the TB and LTBI groups; a different response was detected in QuantiFERON TB-gold test-negative (QFT-) subjects. A significantly different proportion of positive responses was observed between IP-10 and IFN-γ following HBHA stimulation in the TB group and in the QFT- group but not in the LTBI group.

CONCLUSIONS

The IP-10 test seemed to identify false-negative QFT results in some subjects with a positive IFN-γ/IP-10/HBHA pattern.

摘要

背景

本研究旨在评估诱导蛋白-10（IP-10）作为生物标志物，以改善干扰素-γ（IFN-γ）在低结核发病率国家中对活动性结核病（TB）和潜伏性结核感染（LTBI）的识别。

方法

用区域差异 1（RD1）特异性肽和肝素结合血凝素（HBHA）刺激结核分枝杆菌感染受试者的全血，以确定 IP-10 和 IFN-γ的释放。

结果

在 TB 和 LTBI 组中，RD1 特异性肽刺激后 IP-10 和 IFN-γ的阳性率之间未观察到统计学差异；在 QuantiFERON TB-gold 试验阴性（QFT-）受试者中检测到不同的反应。在 TB 组和 QFT-组中，HBHA 刺激后 IP-10 和 IFN-γ的阳性反应比例存在显著差异，但在 LTBI 组中没有差异。