Lau Amy-Sie-Yik, Liong Min-Tze
School of Industrial Technology, Universiti Sains Malaysia, Penang, Malaysia.
School of Industrial Technology, Universiti Sains Malaysia, Penang, Malaysia; email:
Wounds. 2014 May;26(5):121-31.
Lactic acid bacteria (LAB) and bifidobacteria are able to produce antimicrobial compounds to inhibit opportunisticwounding skin pathogen. The antimicrobial compounds produced are organic acids, putative bacteriocin, hydrogen peroxide, and diacetyl. Staphylococcus epidermidis is well-known as an opportunistic wounding skin pathogen in wound infections related to implanted medical devices.
To screen 87 strains of LAB and 3 strains of bifidobacteria for antimicrobial activity against Staphylococcus epidermidis.Additionally, this study sought to determine and quantify types of antimicrobial compounds produced by LAB.
Inhibitory activity of LAB and bifidobacteria on S. epidermidis was assessed with the spectrophotometric method using a 96-well microplate reader. Characterization of cell-free supernatant (CFS) was done using analytical methods. Lactobacillus fermentum (collected by the Bioprocess Department at the Universiti Sains Malaysia [BD]) 1912d, Lactobacillus casei BD 1415b, Lactobacillus fermentum BD 8313a, Pediococcus pentosaceus BD 1913b, and Weissella cibaria (collected by the Food Technology Department at the Universiti Sains Malaysia [FTDC]) 8643 with high percentage of inhibition (P < 0.05), ranging from 73.7% to 88.2%, as compared to the control, were selected for subsequent analyses. Upon neutralization, the antimicrobial activity showed a drastic drop (P < 0.05) in the percentage of inhibition. Concentrations of the inhibitive metabolites were produced in varying amounts and were strain dependent.
Results demonstrated that lactic acid in all strains was produced in a more predominant amount than acetic acid. Protein concentration production ranged from 0.081-0.215 mg/mL. L. fermentum BD 1912d yielded as much as 0.014 mg/mL hydrogen peroxide, which was significantly higher than other strains studied. Diacetyl was produced in a higher concentration by Weissella cibaria FTDC 8643 at 2.884 ng/mL; the lowest concentration of 0.465 ng/mL was produced by Ped. pentosaceus BD 1913b.
Antimicrobial metabolites from CFS of lactic acid bacteria were effective in repressing the growth of opportunistic wounding dermal pathogen Staphylococcus epidermidis.
乳酸菌（LAB）和双歧杆菌能够产生抗菌化合物以抑制机会性伤口皮肤病原体。所产生的抗菌化合物包括有机酸、假定的细菌素、过氧化氢和双乙酰。表皮葡萄球菌是与植入式医疗器械相关的伤口感染中一种众所周知的机会性伤口皮肤病原体。
筛选87株乳酸菌和3株双歧杆菌对表皮葡萄球菌的抗菌活性。此外，本研究旨在确定和量化乳酸菌产生的抗菌化合物类型。
使用96孔微孔板读数器通过分光光度法评估乳酸菌和双歧杆菌对表皮葡萄球菌的抑制活性。使用分析方法对无细胞上清液（CFS）进行表征。发酵乳杆菌（由马来西亚理科大学[BD]生物工艺系收集）1912d、干酪乳杆菌BD 1415b、发酵乳杆菌BD 8313a、戊糖片球菌BD 1913b和西巴韦氏菌（由马来西亚理科大学[FTDC]食品技术系收集）8643与对照相比具有较高的抑制率（P < 0.05），范围为73.7%至88.2%，被选用于后续分析。中和后，抗菌活性的抑制率急剧下降（P < 0.05）。抑制性代谢物的浓度产生量各不相同，且取决于菌株。
结果表明，所有菌株中乳酸的产生量比乙酸更占优势。蛋白质浓度产生范围为0.081 - 0.215 mg/mL。发酵乳杆菌BD 1912d产生的过氧化氢高达0.014 mg/mL显著高于其他研究菌株。西巴韦氏菌FTDC 8643产生的双乙酰浓度较高，为2.884 ng/mL；戊糖片球菌BD 1913b产生的双乙酰浓度最低，为0.465 ng/mL。
乳酸菌CFS中的抗菌代谢物可有效抑制机会性伤口皮肤病原体表皮葡萄球菌的生长。
