Silva Fabrício Souza, Menezes Pedro Modesto Nascimento, de Sá Pedro Guilherme Souza, Oliveira André Luís de Santana, Souza Eric Alencar Araújo, Almeida Jackson Roberto Guedes da Silva, de Lima Julianeli Tolentino, Uetanabaro Ana Paula Trovatti, Silva Tânia Regina dos Santos, Peralta Edna Dória, Lucchese Angélica Maria
a Núcleo de Estudos e Pesquisas em Plantas Medicinais, Colegiado de Ciências Farmacêuticas, Universidade Federal do Vale do São Francisco , Petrolina , Pernambuco , Brazil .
b Programa de Pós-Graduação em Biotecnologia, Departamento de Ciências Biológicas, Universidade Estadual de Feira de Santana , Feira de Santana , Bahia , Brazil .
Pharm Biol. 2016;54(1):25-34. doi: 10.3109/13880209.2015.1005751. Epub 2015 Apr 9.
Lippia thymoides Mart. & Schauer (Verbenaceae) is used in folk medicine to treat wounds, fever, bronchitis, rheumatism, headaches, and weakness.
This study determinates the chemical composition of essential oils from L. thymoides, obtained at during each of the four seasons and correlates with pharmacological properties.
Essential oils were obtained by hydrodistillation and analyzed by gas chromatography coupled to mass spectroscopy (GC-MS). Antioxidant activity was determined by DPPH free radical scavenging and β-carotene bleaching methods. The antimicrobial assays were performed by minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) methods. Isolated rat aorta and uterus, and guinea-pig trachea were utilized to evaluate relaxant potential in pre-contracted smooth muscle.
Essential oils from leaves of L. thymoides had the sesquiterpene β-caryophyllene (17.22-26.27%) as the major constituent followed by borneol (4.45-7.36%), camphor (3.22-8.61%), camphene (2.64-5.66%), and germacrene D (4.72-6.18%). In vitro assays showed that these essential oils do not have antioxidant activity, have antimicrobial selectivity to Gram-positive bacteria Staphylococcus aureus (MIC = 0.004 mg/mL and MMC = 0.26-10.19 mg/mL) and Micrococcus luteus (MIC = 0.03 mg/mL and MMC = 8.43 mg/mL), relax isolated rat aorta (EC50 = 305-544 μg/mL, with endothelium; and EC50 = 150-283 μg/mL, without endothelium), and uterus (EC50 = 74-257 μg/mL), and minor potency, isolated guinea-pig trachea.
Lippia thymoides is a source of natural products of pharmaceutical interest, being necessary additional studies to determine the substances involved in the biological activities.
百里香假马鞭（唇形科）在民间医学中用于治疗伤口、发烧、支气管炎、风湿病、头痛和虚弱。
本研究确定了在四个季节中每个季节获得的百里香假马鞭精油的化学成分，并将其与药理特性相关联。
通过水蒸馏法获得精油，并采用气相色谱 - 质谱联用（GC-MS）进行分析。通过DPPH自由基清除法和β-胡萝卜素漂白法测定抗氧化活性。采用最低抑菌浓度（MIC）和最低杀菌浓度（MMC）法进行抗菌试验。利用离体大鼠主动脉、子宫和豚鼠气管评估对预收缩平滑肌的舒张潜力。
百里香假马鞭叶精油的主要成分是倍半萜β-石竹烯（17.22 - 26.27%），其次是冰片（4.45 - 7.36%）、樟脑（3.22 - 8.61%）、莰烯（2.64 - 5.66%）和杜松烯D（4.72 - 6.18%）。体外试验表明，这些精油没有抗氧化活性，对革兰氏阳性菌金黄色葡萄球菌（MIC = 0.004 mg/mL，MMC = 0.26 - 10.19 mg/mL）和藤黄微球菌（MIC = 0.03 mg/mL，MMC = 8.43 mg/mL）具有抗菌选择性，能舒张离体大鼠主动脉（EC50 = 305 - 544 μg/mL，有内皮；EC50 = 150 - 283 μg/mL，无内皮）和子宫（EC50 = 74 - 257 μg/mL），对离体豚鼠气管的作用较弱。
百里香假马鞭是具有药用价值的天然产物来源，需要进一步研究以确定参与生物活性的物质。
