School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, PR China; Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, PR China.
State Key Laboratory of Food Additive and Condiment Testing, Zhenjiang Entry-Exit Inspection Quarantine Bureau, Zhenjiang 212008, PR China.
Carbohydr Polym. 2015 Jul 10;125:161-8. doi: 10.1016/j.carbpol.2015.02.029. Epub 2015 Feb 21.
Beta-amyloid (Aβ) peptide, the hallmark of Alzheimer's disease, invokes oxidative damage to neurons and eventually leads to neuronal death. Selenylation modification of polysaccharide obtained from Radix hedysari (RHP) was studied to access antioxidant activities and neuroprotective effects against oxidative stress and apoptosis induced by Aβ25-35 in vitro. A series of the selenylation derivatives of RHP (Se-RHP) was synthesized using nitric acid-sodium selenite (HNO3-Na2SeO3) method. The organic selenium content of Se-RHP increased from 1.04 to 3.29 mg/g. However, compared with the weight-average molecular mass (Mw) of RHP, Mw of Se-RHP showed a significant decrease, and varied from 27.7 kDa to 62.7 kDa. FT-IR spectra and (13)C NMR spectra indicated the selenite groups had been introduced mainly at the C-6 positions of RHP. Compared with RHP, Se-RHP showed greater antioxidant activities in vitro. Furthermore, both RHP and Se-RHP3 had neuroprotective effects against Aβ25-35-induced oxidative stress and apoptosis in SH-SY5Y human neuroblastoma cells, which might be a potential therapeutic agent for preventing or treating neurodegenerative diseases.
β-淀粉样肽（Aβ）是阿尔茨海默病的标志性物质，它会引发神经元的氧化损伤，最终导致神经元死亡。本研究旨在探讨从胡芦巴（RHP）中提取的多糖的硒化修饰物的抗氧化活性和神经保护作用，以评估其对 Aβ25-35 诱导的体外氧化应激和细胞凋亡的保护作用。采用硝酸-亚硒酸钠（HNO3-Na2SeO3）法合成了一系列 RHP 的硒化衍生物（Se-RHP）。Se-RHP 的有机硒含量从 1.04 增加到 3.29 mg/g。然而，与 RHP 的重均分子量（Mw）相比，Se-RHP 的 Mw 显著降低，范围从 27.7 kDa 到 62.7 kDa。FT-IR 光谱和（13）C NMR 光谱表明，硒代亚硫酸盐基团主要被引入 RHP 的 C-6 位。与 RHP 相比，Se-RHP 在体外具有更强的抗氧化活性。此外，RHP 和 Se-RHP3 对 Aβ25-35 诱导的 SH-SY5Y 人神经母细胞瘤细胞氧化应激和细胞凋亡均具有神经保护作用，这可能使其成为预防或治疗神经退行性疾病的潜在治疗药物。
