Chen Ling, Wang Lu, Sheng Xia-Fang
Key Laboratory of Agricultural Environment Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
Int J Syst Evol Microbiol. 2015 Jul;65(7):2161-2166. doi: 10.1099/ijs.0.000232. Epub 2015 Apr 9.
A novel bacterial strain, S1-9(T), was isolated from a lead-zinc tailing in Nanjing, Jiangsu Province, China. Cells of strain S1-9(T) were Gram-stain-negative, ellipsoidal endospore-forming, aerobic rods and motile by means of peritrichous flagella. On the basis of 16S rRNA gene sequence analysis, strain S1-9(T) was shown to belong to the genus Paenibacillus and the closest phylogenetic relatives were Paenibacillus glucanolyticus DSM 5162(T) (96.8% similarity), Paenibacillus lautus NRRL NRS-666(T) (96.5%) and Paenibacillus lactis MB 1871(T) (95.4%). The predominant menaquinone was MK-7. The major cellular fatty acids were anteiso-C15:0 and iso-C16:0. The polar lipid profile contained phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, two unknown phospholipids and two unknown lipids. The total DNA G+C content of strain S1-9(T) was 49.9 mol%. Based on the low levels of DNA-DNA relatedness (ranging from 21.8 to 48.4%) to the type strains of the above species of the genus Paenibacillus and unique phenotypic characteristics, strain S1-9(T) is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus qingshengii sp. nov. is proposed. The type strain is S1-9(T) ( = CCTCC AB 2014290(T) = JCM 30613(T)).
从中国江苏省南京市的铅锌尾矿中分离出一株新型细菌菌株S1-9(T)。菌株S1-9(T)的细胞革兰氏染色阴性，形成椭圆形内生孢子，为需氧杆菌，借助周生鞭毛运动。基于16S rRNA基因序列分析，菌株S1-9(T)被证明属于芽孢杆菌属，与其亲缘关系最近的系统发育相关菌株为解葡聚糖芽孢杆菌DSM 5162(T)（相似度96.8%）、迟缓芽孢杆菌NRRL NRS-666(T)（96.5%）和乳酸芽孢杆菌MB 1871(T)（95.4%）。主要的甲基萘醌为MK-7。主要的细胞脂肪酸为anteiso-C15:0和iso-C16:0。极性脂质谱包含磷脂酰甘油、磷脂酰乙醇胺、二磷脂酰甘油、两种未知磷脂和两种未知脂质。菌株S1-9(T)的总DNA G+C含量为49.9 mol%。基于与芽孢杆菌属上述物种模式菌株的低水平DNA-DNA相关性（范围为21.8%至48.4%）以及独特的表型特征，菌株S1-9(T)被认为代表芽孢杆菌属的一个新物种，为此提出新名称青生芽孢杆菌（Paenibacillus qingshengii sp. nov.）。模式菌株为S1-9(T)（=CCTCC AB 2014290(T)=JCM 30613(T)）。
