Joseph Betsy, Prasanth Chandra Sekhar, Jayanthi Jayaraj L, Presanthila Janam, Subhash Narayanan
Government Dental College, Department of Periodontics, Medical College P.O., Thiruvananthapuram 695 011, Kerala, India.
National Centre for Earth Science Studies, Biophotonics Laboratory, Akkulam, Thiruvananthapuram 695031, Kerala, IndiacUniversity of Washington, Department of Mechanical Engineering, Seattle, Washington 98195, United States.
J Biomed Opt. 2015 Apr;20(4):048001. doi: 10.1117/1.JBO.20.4.048001.
The aim of this study was to evaluate the applicability of laser-induced autofluorescence (LIAF) spectroscopy to detect and quantify dental plaque. LIAF spectra were recorded in situ from dental plaque (0–3 grades of plaque index) in 300 patients with 404 nm diode laser excitation. The fluorescence intensity ratio of the emission peaks was calculated from the LIAF spectral data following which their scatter plots were drawn and the area under the receiver operating characteristics were calculated. The LIAF spectrum of clinically invisible grade-1 plaque showed a prominent emission peak at 510 nm with a satellite peak around 630 nm in contrast to grade 0 that has a single peak around 500 nm. The fluorescence intensity ratio (F510/F630) has a decreasing trend with increase in plaque grade and the ratio values show statistically significant differences (p<0.01) between different grades. An overall sensitivity and specificity of 100% each was achieved for discrimination between grade-0 and grade-1 plaque. The clinical significance of this study is that the diagnostic algorithm developed based on fluorescence spectral intensity ratio (F510/F630) would be useful to precisely identify minute amounts of plaque without the need for disclosing solutions and to convince patients of the need for proper oral hygiene and homecare practices.
本研究的目的是评估激光诱导自体荧光（LIAF）光谱技术在检测和量化牙菌斑方面的适用性。采用404nm二极管激光激发，对300例患者的牙菌斑（菌斑指数0 - 3级）进行原位记录LIAF光谱。根据LIAF光谱数据计算发射峰的荧光强度比，绘制散点图并计算受试者工作特征曲线下面积。与0级牙菌斑在500nm左右有一个单峰相比，临床不可见的1级牙菌斑的LIAF光谱在510nm处有一个突出的发射峰，在630nm左右有一个卫星峰。荧光强度比（F510/F630）随菌斑等级增加呈下降趋势，不同等级之间的比值差异具有统计学意义（p<0.01）。在区分0级和1级牙菌斑时，总体灵敏度和特异性均达到100%。本研究的临床意义在于，基于荧光光谱强度比（F510/F630）开发的诊断算法将有助于在无需使用显影剂的情况下精确识别微量牙菌斑，并使患者认识到保持良好口腔卫生和家庭护理的必要性。
