Planas Oriol, Bresolí-Obach Roger, Nos Jaume, Gallavardin Thibault, Ruiz-González Rubén, Agut Montserrat, Nonell Santi
Institut Químic de Sarrià, Universitat Ramon Llull, Via Augusta 390, 08017 Barcelona, Spain.
Molecules. 2015 Apr 9;20(4):6284-98. doi: 10.3390/molecules20046284.
Over the last 20 years, the number of pathogenic multi-resistant microorganisms has grown steadily, which has stimulated the search for new strategies to combat antimicrobial resistance. Antimicrobial photodynamic therapy (aPDT), also called photodynamic inactivation, is emerging as a promising alternative to treatments based on conventional antibiotics. We have explored the effectiveness of methylene blue-loaded targeted mesoporous silica nanoparticles (MSNP) in the photodynamic inactivation of two Gram negative bacteria, namely Escherichia coli and Pseudomonas aeruginosa. For E. coli, nanoparticle association clearly reduced the dark toxicity of MB while preserving its photoinactivation activity. For P. aeruginosa, a remarkable difference was observed between amino- and mannose-decorated nanoparticles. The details of singlet oxygen production in the nanoparticles have been characterized, revealing the presence of two populations of this cytotoxic species. Strong quenching of singlet oxygen within the nanoparticles is observed.
在过去20年中，致病性多重耐药微生物的数量稳步增长，这促使人们寻找对抗抗菌药物耐药性的新策略。抗菌光动力疗法（aPDT），也称为光动力失活，正作为一种有前途的替代传统抗生素治疗的方法而兴起。我们研究了负载亚甲蓝的靶向介孔二氧化硅纳米颗粒（MSNP）对两种革兰氏阴性菌，即大肠杆菌和铜绿假单胞菌的光动力失活效果。对于大肠杆菌，纳米颗粒结合明显降低了亚甲蓝的暗毒性，同时保留了其光灭活活性。对于铜绿假单胞菌，在氨基修饰和甘露糖修饰的纳米颗粒之间观察到显著差异。已经对纳米颗粒中单线态氧产生的细节进行了表征，揭示了这种细胞毒性物质存在两个群体。观察到纳米颗粒内单线态氧的强烈猝灭。
