Alencar Daniel B, Melo Arthur A, Silva Giselle C, Lima Rebeca L, Pires-Cavalcante Kelma M S, Carneiro Rômulo F, Rabelo Adriana S, Sousa Oscarina V, Vieira Regine H S F, Viana Francisco A, Sampaio Alexandre H, Saker-Sampaio Silvana
Departamento de Engenharia de Pesca, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
Instituto de Ciências do Mar, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
An Acad Bras Cienc. 2015 Apr-Jun;87(2):1113-23. doi: 10.1590/0001-3765201520140370. Epub 2015 Apr 3.
Marine invertebrates are capable of synthesizing bioactive compounds, which may be beneficial to human health. The aim of this study was to evaluate the antioxidant, hemolytic, antimicrobial and cytotoxic activities of crude extract (70% EtOH), and dichloromethane (DCM), ethyl acetate (EtOAc), and aqueous (Aq) fractions of the marine zoanthid Palythoa caribaeorum. The phenolic compound contents of the crude extract, DCM, EtOAc and Aq fractions were 12.33, 18.17, 10.53, and 3.18 mg GAE per gram, respectively. DPPH radical scavenging activity showed slight variation. IC50 of crude extract, DCM, EtOAc and Aq fractions were 11.13, 11.25, 11.74, and 11.28 µg mL(-1), respectively. Among the sample, ferrous ion chelating was the highest in crude extract (IC50 302.90 µg mL(-1)), followed by EtOAc, Aq, and DCM fractions with 457.77, 547.91, and 641.82 µg mL(-1), respectively. Ferric-reducing antioxidant power showed optical density at about 0.5. The samples tested exhibited low hemolytic activity under 10% up to a concentration of 50 μg mL(-1). No antimicrobial activity was observed against any of the tested bacterial strains. For the cytotoxic activity, LC50 of DCM, crude extract, EtOAc, and Aq were 52.10, 83.06, 86.34, and 117.45 μg mL(-1), showing high toxicity.
海洋无脊椎动物能够合成对人类健康有益的生物活性化合物。本研究的目的是评估海洋珊瑚虫加勒比绿须软珊瑚（Palythoa caribaeorum）的粗提物（70%乙醇）以及二氯甲烷（DCM）、乙酸乙酯（EtOAc）和水相（Aq）组分的抗氧化、溶血、抗菌和细胞毒性活性。粗提物、DCM、EtOAc和Aq组分的酚类化合物含量分别为每克12.33、18.17、10.53和3.18毫克没食子酸当量。DPPH自由基清除活性略有变化。粗提物、DCM、EtOAc和Aq组分的IC50分别为11.13、11.25、11.74和11.28微克/毫升。在这些样品中，粗提物的亚铁离子螯合能力最强（IC50为302.90微克/毫升），其次是EtOAc、Aq和DCM组分，IC50分别为457.77、547.91和641.82微克/毫升。铁还原抗氧化能力显示在约0.5处的光密度。测试的样品在浓度高达50微克/毫升时，溶血活性低于10%。未观察到对任何测试细菌菌株的抗菌活性。对于细胞毒性活性，DCM、粗提物、EtOAc和Aq的LC50分别为52.10、83.06、86.34和117.45微克/毫升，显示出高毒性。
