Research Center of Supercritical Fluid Technology, Tohoku University, Aoba-ku, Sendai, Japan.
Bioresour Technol. 2011 Feb;102(3):3286-92. doi: 10.1016/j.biortech.2010.10.024. Epub 2010 Oct 12.
Effects of oxygen and nitrate on fatty acid/lipid production from a highly CO(2)-tolerant microalgal species Chlorococcum littorale were examined under photoautotrophic conditions of 295 K, a light intensity of 170 μmol-photon m(-2) s(-1), a bubbling CO(2) concentration of 5% (v/v) and bubbling oxygen concentrations to be volumetrically adjusted by mixing oxygen gas with inert nitrogen gas at concentrations ranging from 0% to 95% (v/v). The results showed that maximum fatty acid content reached ca. 34 wt.% under oxygen-freely bubbling conditions and this value decreased to be ca. 20 wt.% when air-like oxygen concentration of 20% was chosen. This means that degree of the accumulation strongly depended on the level of bubbling oxygen concentrations, which can be a crucial factor after nitrogen depletion in the photoautotrophic culture system. TLC-FID/FPD analyses showed that triglycerides were found to be a dominant lipid class for this accumulation.
在 295 K、光照强度为 170 μmol-photon m(-2) s(-1)、5%（v/v）鼓泡 CO(2)浓度和通过混合氧气和惰性氮气来在 0%至 95%（v/v）体积比范围内调节鼓泡氧气浓度的光自养条件下，研究了氧气和硝酸盐对高度耐受 CO(2)的微藻种 Chlorococcum littorale 的脂肪酸/脂质生产的影响。结果表明，在无氧气鼓泡条件下，最大脂肪酸含量达到约 34wt%，而当选择类似空气的 20%氧气浓度时，该值降低至约 20wt%。这意味着积累程度强烈取决于鼓泡氧气浓度水平，这在光自养培养系统中氮耗尽后可能是一个关键因素。TLC-FID/FPD 分析表明，三酰基甘油是这种积累的主要脂质类别。
