Lieberman H B, Riley R, Martel M
Center for Radiological Research, College of Physicians and Surgeons, Columbia University, New York, NY 10032.
Mol Gen Genet. 1989 Sep;218(3):554-8. doi: 10.1007/BF00332423.
We have isolated a mutant of the yeast Schizosaccharomyces pombe which exhibits sensitivity to UV light when grown at either 30 degrees or 37 degrees C, as compared to the parental wild-type strain. This increased sensitivity is more pronounced when cells are grown at 37 degrees C. The mutant is also sensitive to 18 MeV electrons at the high temperature. Tetrad analysis of spores generated by crossing the mutant and a Rad+ strain revealed that sensitivity to both types of radiation cosegregate 2:2, relative to wild-type resistance, indicating that a single altered chromosomal locus is responsible for the radiation sensitivities observed. In addition, analysis of spores resulting from crosses between the mutant and all other known S. pombe rad mutants indicates that the temperature-dependent sensitivity described in this report is mediated by a mutation in a previously unidentified rad locus.
我们分离出了粟酒裂殖酵母的一个突变体，与亲本野生型菌株相比，该突变体在30℃或37℃生长时对紫外线敏感。当细胞在37℃生长时，这种增加的敏感性更为明显。该突变体在高温下对18 MeV电子也敏感。将该突变体与Rad+菌株杂交产生的孢子进行四分体分析，结果表明，相对于野生型抗性，对两种辐射的敏感性以2:2共分离，这表明单个改变的染色体位点导致了所观察到的辐射敏感性。此外，对该突变体与所有其他已知的粟酒裂殖酵母rad突变体杂交产生的孢子进行分析表明，本报告中描述的温度依赖性敏感性是由一个以前未鉴定的rad位点的突变介导的。
