粟酒裂殖酵母的温度敏感突变体表现出增强的辐射敏感性。
Temperature-sensitive mutant of Schizosaccharomyces pombe exhibiting enhanced radiation sensitivity.
作者信息
Duck P, Nasim A, James A P
出版信息
J Bacteriol. 1976 Nov;128(2):536-9. doi: 10.1128/jb.128.2.536-539.1976.
Abstract
A conditional lethal and radiation-sensitive mutant of Schizosaccharomyces pombe is described in which both characteristics result from a single gene mutation. Confirmation of the pleiotropic nature of this mutant was obtained by tetrad analysis and by testing the radiation sensitivity of a large number of revertants that grew normally at the restrictive temperature. The colony-forming ability of the mutant after ultraviolet radiation, gamma radiation, and ethyl methane sulfonate treatment is considerably altered by the post-treatment incubation temperature, showing higher survival at 25 than at 30degreesC. The radiosensitivity of the mutant is also influenced by the stage of growth. The difference in radiation sensitivity between the wild type and mutant is greater when log-phase cultures are compared. The characteristics of this mutant suggest that it is defective in a step common to both deoxyribonucleic acid replication and repair.
摘要
描述了粟酒裂殖酵母的一种条件致死且对辐射敏感的突变体，其中这两种特性均由单一基因突变所致。通过四分体分析以及测试大量在限制温度下正常生长的回复体的辐射敏感性，证实了该突变体的多效性。紫外线辐射、γ辐射和甲磺酸乙酯处理后，该突变体的集落形成能力会因处理后的培养温度而发生显著改变，在25℃时的存活率高于30℃。该突变体的辐射敏感性也受生长阶段影响。比较对数期培养物时，野生型和突变体之间的辐射敏感性差异更大。此突变体的特性表明，它在脱氧核糖核酸复制和修复的一个共同步骤中存在缺陷。
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