Media Lab, Massachusetts Institute of Technology, 75 Amherst Street, Cambridge, Massachusetts 02139, USA.
Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
Nat Commun. 2015 Apr 13;6:6796. doi: 10.1038/ncomms7796.
The use of fluorescent probes and the recovery of their lifetimes allow for significant advances in many imaging systems, in particular, medical imaging systems. Here we propose and experimentally demonstrate reconstructing the locations and lifetimes of fluorescent markers hidden behind a turbid layer. This opens the door to various applications for non-invasive diagnosis, analysis, flowmetry and inspection. The method is based on a time-resolved measurement that captures information about both fluorescence lifetime and spatial position of the probes. To reconstruct the scene, the method relies on a sparse optimization framework to invert time-resolved measurements. This wide-angle technique does not rely on coherence, and does not require the probes to be directly in line of sight of the camera, making it potentially suitable for long-range imaging.
荧光探针的使用以及寿命的恢复使得许多成像系统，尤其是医学成像系统得到了显著的发展。在这里，我们提出并实验证明了可以重建隐藏在混浊层后面的荧光标记物的位置和寿命。这为非侵入性诊断、分析、流量测量和检查等各种应用打开了大门。该方法基于时间分辨测量，可获取探针的荧光寿命和空间位置信息。为了重建场景，该方法依赖于稀疏优化框架来反转时间分辨测量。这种广角技术不依赖于相干性，也不需要探针直接在相机的视线内，因此可能适合远程成像。
