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非洲爪蟾核糖体蛋白L5的发育表达及5S rRNA结合活性

Developmental expression and 5S rRNA-binding activity of Xenopus laevis ribosomal protein L5.

作者信息

Wormington W M

机构信息

Department of Biology, University of Virginia, Charlottesville 22901.

出版信息

Mol Cell Biol. 1989 Dec;9(12):5281-8. doi: 10.1128/mcb.9.12.5281-5288.1989.

DOI:10.1128/mcb.9.12.5281-5288.1989
PMID:2586520
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC363692/
Abstract

Ribosomal protein L5 binds specifically to 5S rRNA to form a complex that is a precursor to 60S subunit assembly in vivo. Analyses in yeast cells, mammalian cells, and Xenopus embryos have shown that the accumulation of L5 is not coordinated with the expression of other ribosomal proteins. In this study, the primary structure and developmental expression of Xenopus ribosomal protein L5 were examined to determine the basis for its distinct regulation. These analyses showed that L5 expression could either coincide with 5S rRNA synthesis and ribosome assembly or be controlled independently of these events at different stages of Xenopus development. L5 synthesis during oogenesis was uncoupled from the accumulation of 5S rRNa but coincided with subunit assembly. In early embryos, the inefficient translation of L5 mRNA resulted in the accumulation of a stable L5-5S rRNA complex before ribosome assembly at later stages of development. Additional results demonstrated that L5 protein synthesized in vitro bound specifically to 5S rRNA.

摘要

核糖体蛋白L5特异性结合5S rRNA，形成一种复合物，该复合物是体内60S亚基组装的前体。对酵母细胞、哺乳动物细胞和非洲爪蟾胚胎的分析表明，L5的积累与其他核糖体蛋白的表达并不协调。在本研究中，对非洲爪蟾核糖体蛋白L5的一级结构和发育表达进行了研究，以确定其独特调控的基础。这些分析表明，L5的表达可能与5S rRNA合成和核糖体组装同时发生，或者在非洲爪蟾发育的不同阶段独立于这些事件进行调控。卵子发生过程中L5的合成与5S rRNA的积累不相关，但与亚基组装同时发生。在早期胚胎中，L5 mRNA的低效翻译导致在发育后期核糖体组装之前积累稳定的L5-5S rRNA复合物。其他结果表明，体外合成的L5蛋白特异性结合5S rRNA。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/165c/363692/80262e658fa2/molcellb00060-0022-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/165c/363692/6fb454e09e1e/molcellb00060-0020-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/165c/363692/d5f8efe61c86/molcellb00060-0021-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/165c/363692/80262e658fa2/molcellb00060-0022-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/165c/363692/6fb454e09e1e/molcellb00060-0020-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/165c/363692/d5f8efe61c86/molcellb00060-0021-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/165c/363692/80262e658fa2/molcellb00060-0022-a.jpg

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