Li Yingju, Sun Xiaofei, Dey Sudhansu K
Division of Reproductive Sciences, Cincinnati Children's Research Foundation, Cincinnati, OH 45229, USA.
Division of Reproductive Sciences, Cincinnati Children's Research Foundation, Cincinnati, OH 45229, USA.
Cell Rep. 2015 Apr 21;11(3):358-65. doi: 10.1016/j.celrep.2015.03.035. Epub 2015 Apr 9.
During implantation, uterine luminal epithelial (LE) cells first interact with the blastocyst trophectoderm. Within 30 hr after the initiation of attachment, LE cells surrounding the blastocyst in the implantation chamber (crypt) disappear, allowing trophoblast cells to make direct physical contact with the underneath stroma for successful implantation. The mechanism for the extraction of LE cells was thought to be mediated by apoptosis. Here, we show that LE cells in direct contact with the blastocyst are endocytosed by trophoblast cells by adopting the nonapoptotic cell-in-cell invasion process (entosis) in the absence of caspase 3 activation. Our in vivo observations were reinforced by the results of co-culture experiments with primary uterine epithelial cells with trophoblast stem cells or blastocysts showing internalization of epithelial cells by trophoblasts. We have identified entosis as a mechanism to remove LE cells by trophoblast cells in implantation, conferring a role for entosis in an important physiological process.
在植入过程中，子宫腔上皮（LE）细胞首先与囊胚滋养外胚层相互作用。在附着开始后的30小时内，植入腔（隐窝）中围绕囊胚的LE细胞消失，使滋养层细胞能够与下方的基质直接进行物理接触，从而实现成功植入。LE细胞的清除机制被认为是由凋亡介导的。在这里，我们表明，在没有半胱天冬酶3激活的情况下，与囊胚直接接触的LE细胞通过采用非凋亡性的细胞内细胞侵袭过程（细胞内吞作用）被滋养层细胞内吞。用原代子宫上皮细胞与滋养层干细胞或囊胚进行共培养实验的结果强化了我们的体内观察结果，该实验显示滋养层细胞将上皮细胞内化。我们已经确定细胞内吞作用是植入过程中滋养层细胞清除LE细胞的一种机制，赋予了细胞内吞作用在一个重要生理过程中的作用。
