Dexter J, Armshaw P, Sheahan C, Pembroke J T
Molecular and Structural Biochemistry Laboratory, Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland.
Department of Design and Manufacturing Engineering, University of Limerick, Limerick, Ireland.
J Appl Microbiol. 2015 Jul;119(1):11-24. doi: 10.1111/jam.12821. Epub 2015 May 19.
Ethanol production directly from CO2 , utilizing genetically engineered photosynthetic cyanobacteria as a biocatalyst, offers significant potential as a renewable and sustainable source of biofuel. Despite the current absence of a commercially successful production system, significant resources have been deployed to realize this goal. Utilizing the pyruvate decarboxylase from Zymomonas species, metabolically derived pyruvate can be converted to ethanol. This review of both peer-reviewed and patent literature focuses on the genetic modifications utilized for metabolic engineering and the resultant effect on ethanol yield. Gene dosage, induced expression and cassette optimizat-ion have been analyzed to optimize production, with production rates of 0·1-0·5 g L(-1) day(-1) being achieved. The current 'toolbox' of molecular manipulations and future directions focusing on applicability, addressing the primary challenges facing commercialization of cyanobacterial technologies are discussed.
利用基因工程改造的光合蓝细菌作为生物催化剂，直接从二氧化碳生产乙醇，作为一种可再生且可持续的生物燃料来源具有巨大潜力。尽管目前尚无商业上成功的生产系统，但已投入大量资源来实现这一目标。利用来自发酵单胞菌属的丙酮酸脱羧酶，代谢产生的丙酮酸可转化为乙醇。这篇对同行评审文献和专利文献的综述聚焦于代谢工程所采用的基因改造及其对乙醇产量的影响。已对基因剂量、诱导表达和盒式优化进行了分析以优化生产，实现的生产率为0·1 - 0·5克/升·天(-1)。讨论了当前分子操作的“工具箱”以及关注适用性的未来方向，以应对蓝细菌技术商业化面临的主要挑战。
