一种改良的染料倾注平板生长谱法用于快速鉴定临床重要酵母菌的评估。与两种商业系统的比较。

Evaluation of a modified dye pour-plate auxanographic method for the rapid identification of clinically significant yeasts. Comparison with two commercial systems.

作者信息

Weymann L H, Stager C E, Qadri S G, Villarreal A, Qadri S M

出版信息

Med Microbiol Immunol. 1979 Mar 13;167(1):11-20. doi: 10.1007/BF02123291.

Abstract

A modified dye pour-plate auxanographic (DPPA) method for the presumptive identification of medically important yeasts was evaluated, in a comparative study with a conventional procedure, the API 20C clinical yeast system (Analytab Products Inc.), and the Uni-Yeast-Tek (UYT) system. The 174 coded clinical isolates were members of the genera Candida, Cryptococcus, Rhodotorula, Saccharomyces, Torulopsis, and Trichosporon. The identification accuracies with DPPA, API, and UYT were 95%, 93%, and 99% respectively. DPPA and API required more time to inoculate but gave rapid identification profiles. UYT was simple to inoculate and both UYT and DPPA were easy to read. Cost analysis of the three rapid methods demonstrated DPPA to be the most economical making it a feasible alternative for small clinical laboratories as well as large laboratories possessing the facilities to make their own media.

摘要

对一种用于初步鉴定重要医学酵母的改良染料倾注平板生长谱法（DPPA）进行了评估，将其与传统方法、API 20C临床酵母系统（Analytab Products公司）以及Uni-Yeast-Tek（UYT）系统进行了比较研究。174株编码临床分离株属于念珠菌属、隐球菌属、红酵母属、酿酒酵母属、球拟酵母属和丝孢酵母属。DPPA、API和UYT的鉴定准确率分别为95%、93%和99%。DPPA和API接种所需时间更长，但能给出快速的鉴定结果。UYT接种简单，UYT和DPPA的结果都易于判读。对这三种快速方法的成本分析表明，DPPA最经济，这使其成为小型临床实验室以及具备自制培养基设施的大型实验室的可行替代方法。