Balasingham K, Valli C, Radhakrishnan L, Balasuramanyam D
Postgraduate Research Institute of Animal Sciences, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu, India.
Institute of Animal Nutrition, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu, India.
Vet World. 2017 Jul;10(7):825-829. doi: 10.14202/vetworld.2017.825-829. Epub 2017 Jul 27.
A study was conducted with the objective to isolate probiotic microorganisms from swine intestine.
In this study 63 isolates (24 caeca, 24 colon mucosal scrapings, and 15 rectal swab samples) were collected from Large White Yorkshire pigs. The isolates were inoculated and grown in de Man Rogosa Sharpe broth at 37°C with 5% CO for 48 h and subjected to morphological identification. Colonies having Gram-positive rods were selected for further physiological and biochemical identification tests, which were conducted in triplicate in two runs for each of the selected isolates using a standard protocol. Probiotic properties among the identified species were determined through the implementation of several tests related with pH tolerance, bile tolerance, and antimicrobial activity.
Morphological identification revealed that only 23 isolates were Gram-positive rods. Physiological tests performed on these 23 isolates further revealed that four of them did not exhibit any growth, at all conditions studied. The rest 19 isolates were, therefore, selected and subjected to biochemical tests. Six isolates were rejected because they were oxidase and nitrate reduction positive. From the 13 isolates subjected to sugar fermentation tests, speciation of only two isolates could be ascertained, one of the isolates showed characteristics for and the other for . These two isolates were assessed for the strain possessing maximum probiotic property, and it was inferred that both - and could tolerate a wide pH range (2-9), a wide bile concentration (0.05-0.3%) and revealed antimicrobial activity toward , and spp.
and were isolated from swine intestine and were found to have good probiotic properties.
进行一项研究以从猪肠道中分离益生菌微生物。
在本研究中，从大白约克夏猪收集了63株分离菌（24株盲肠菌、24株结肠黏膜刮取物和15株直肠拭子样本）。将分离菌接种于德氏罗格斯夏普肉汤中，在37°C、5%二氧化碳条件下培养48小时，并进行形态学鉴定。选择革兰氏阳性杆菌菌落进行进一步的生理生化鉴定试验，对每个选定的分离菌按照标准方案分两次进行一式三份的试验。通过实施与耐pH值、耐胆汁和抗菌活性相关的多项试验来确定已鉴定物种中的益生菌特性。
形态学鉴定显示只有23株分离菌为革兰氏阳性杆菌。对这23株分离菌进行的生理试验进一步表明，其中4株在所有研究条件下均未生长。因此，其余19株分离菌被选中并进行生化试验。6株分离菌因氧化酶和硝酸盐还原呈阳性而被排除。在进行糖发酵试验的13株分离菌中，仅能确定2株分离菌的种类，其中一株显示出[具体种类1]的特征而另一株显示出[具体种类2]的特征。对这两株分离菌进行具有最大益生菌特性菌株的评估，推断[具体种类1]和[具体种类2]均能耐受较宽的pH范围（2 - 9）、较宽的胆汁浓度（0.05 - 0.3%），并对[具体菌种1]、[具体菌种2]等显示出抗菌活性。
[具体种类1]和[具体种类2]从猪肠道中分离得到，且被发现具有良好的益生菌特性。
