Arisha M H, Liang B-K, Muhammad Shah S N, Gong Z-H, Li D-W
College of Horticulture, Northwest A&F University, Yangling, Shaanxi, China.
Seed Administration Station of Jintai District, Baoji, Shaanxi, China.
Genet Mol Res. 2014 Nov 28;13(4):10049-61. doi: 10.4238/2014.November.28.9.
Pepper seeds (Capsicum annuum L.) var. B12 were mutagenized by four presoaking treatments in ten concentrations of ethyl methane sulfonate (EMS) to determine the sensitivity of the first generation (M1) to mutagens. The spectrum of mutations and induced variability for various quantitative traits, including germination, percent plant height, injury occurrence, survival ratio, first three fruits weight, and number of seeds per first fruit, were observed in the M1 generation. Our results indicated that all of the test parameters decreased with increasing EMS concentration, except for seedling injury. There were significant differences in germination ratio, LD50, plant height, percent injury, and survival ratio among the tested presoaking treatment. The LD50 was 1% EMS in seeds that were not presoaked (T1) and seeds presoaked for 12 h before treating with EMS (T3). In contrast, the LD50 was 0.5% EMS in seeds presoaked for 6 h (T2) and seeds presoaked in water for 6 h then incubated at 28°C for 12 h before EMS treatment (T4). Five dwarf plants were observed in mutagenized seeds without presoaking as compared to control seeds (at the maturity stage of the control plant).
辣椒种子（Capsicum annuum L.）品种B12通过在十种浓度的甲基磺酸乙酯（EMS）中进行四种预浸泡处理进行诱变，以确定第一代（M1）对诱变剂的敏感性。在M1代中观察了各种数量性状的突变谱和诱导变异性，包括发芽率、株高百分比、损伤发生率、存活率、前三个果实重量以及第一个果实的种子数。我们的结果表明，除了幼苗损伤外，所有测试参数均随着EMS浓度的增加而降低。在所测试的预浸泡处理中，发芽率、LD50、株高、损伤百分比和存活率存在显著差异。未预浸泡的种子（T1）和在EMS处理前预浸泡12小时的种子（T3）的LD50为1% EMS。相比之下，预浸泡6小时的种子（T2）和在水中预浸泡6小时然后在28°C下孵育12小时再进行EMS处理的种子（T4）的LD50为0.5% EMS。与对照种子相比（在对照植物成熟阶段），在未预浸泡的诱变种子中观察到五株矮化植株。
