Ipek M, Seker M, Ipek A, Gul M K
Department of Horticulture, Faculty of Agriculture, Uludag University, Bursa, Turkey
Department of Horticulture, Faculty of Agriculture, Sırnak University, Sırnak, Turkey.
Genet Mol Res. 2015 Mar 31;14(1):2762-74. doi: 10.4238/2015.March.31.6.
The purpose of this study was to characterize olive core collection with amplified fragment length polymorphism (AFLP) markers and fruit traits and to determine AFLP markers significantly associated with these fruit characters in olive. A total of 168 polymorphic AFLP markers generated by five primer combinations and nine fruit traits were used to characterize relationships between 18 olive cultivars. Although all olive cultivars were discriminated from each other by either AFLP markers (<0.75 similarity level) or fruit traits, clustering based on the AFLP markers and fruit traits was not significantly correlated (r = 0.13). Partial clustering of olive cultivars by AFLP markers according to their geographical origin was observed. Associations of AFLP markers with fruits were determined using a multiple-regression analysis with stepwise addition of AFLP markers. Significant associations between eight AFLP markers and fruit traits were identified. While five AFLP markers demonstrated significant negative correlation with fruit and stone weight, width and length and total polyphenols (P < 0.05), three AFLP markers displayed significant positive correlation with α-tocopherol and γ-tocopherol (P < 0.01). This is the first report on the association of molecular markers with fruit traits in olive. Molecular markers associated with morphological and agronomic traits could be utilized for the breeding of olive cultivars. However, the association power of these markers needs to be confirmed in larger populations, and highly correlated markers should then be converted to PCR-based DNA markers such as sequence-characterized amplified region markers for better utilization.
本研究的目的是利用扩增片段长度多态性（AFLP）标记和果实性状对油橄榄核心种质进行特征描述，并确定与油橄榄这些果实性状显著相关的AFLP标记。使用由五种引物组合产生的总共168个多态性AFLP标记和九个果实性状来描述18个油橄榄品种之间的关系。尽管所有油橄榄品种都可通过AFLP标记（相似性水平<0.75）或果实性状相互区分，但基于AFLP标记和果实性状的聚类并不显著相关（r = 0.13）。观察到根据地理起源，油橄榄品种通过AFLP标记进行了部分聚类。使用逐步添加AFLP标记的多元回归分析来确定AFLP标记与果实的关联。鉴定出八个AFLP标记与果实性状之间存在显著关联。五个AFLP标记与果实和果核重量、宽度和长度以及总多酚呈显著负相关（P < 0.05），三个AFLP标记与α-生育酚和γ-生育酚呈显著正相关（P < 0.01）。这是关于油橄榄分子标记与果实性状关联的首次报道。与形态学和农艺性状相关的分子标记可用于油橄榄品种的育种。然而，这些标记的关联能力需要在更大的群体中得到证实，然后应将高度相关的标记转化为基于PCR的DNA标记，如序列特征扩增区域标记，以便更好地利用。
