Department of Crop and Animal Production, Organic Agriculture Programme, Karacabey Vocational School, Bursa Uludağ University, Bursa, Turkey.
Genetic Resources Institute of Azerbaijan, National Academy of Sciences, Baku, Azerbaijan.
Mol Biol Rep. 2022 Jun;49(6):5209-5217. doi: 10.1007/s11033-021-06564-x. Epub 2021 Jul 21.
Olive (Olea europaea L.) is one of the most economically important crop from east to the west around the world. The aim of this research was to investigate the genetic relationship among 41 olive genotypes, including 11 well-known Turkish cultivars and 30 Azerbaijani olive genotypes using simple sequence repeat (SSR) markers. In this study, 19 SSR markers were amplified 115 polymorphic SSR alleles. The number of polymorphic alleles ranged from 3 to 10 with an average of 6.05. The observed heterozygosity (Ho) varied from 0.05 to 0.93 with an average of 0.63 and expected heterozygosity (He) differed from 0.26 to 0.86 with an average of 0.72. The polymorphism information content (PIC) ranged from 0.23 to 0.85 with a mean of 0.68. A UPGMA cluster analysis grouped olive genotypes into two distinct clusters and both clusters were divided into two subgroups. Similarly, STRUCTURE analysis assigned olive genotypes into two different gene pools (K = 2) and four gene pools were identified representing the two subgroups by STRUCTURE analysis for K = 4. The genetic similarity of olive genotypes ranged from 0.36 to 0.95. These results revealed that there was a high genetic variation among 30 Azerbaijani olive genotypes. 'Ayvalık 1'and 'Ayvalık 2' from Azerbaijani olive genotypes were different from Turkish local olive cultivar, "Ayvalık" indicating homonymy. This research also highlighted that Azerbaijani olive genotypes were totally distinct from Turkish olive cultivars demonstrating that these olive genotypes might have been imported to Azerbaijan from different countries other than Turkey. The outcomes of this study indicated that these diverse olive genotypes could be useful for development of new olive varieties in Azerbaijan and future breeding programs between two countries could be enhanced by means of these results.
橄榄（Olea europaea L.）是世界范围内从东到西最具经济重要性的作物之一。本研究旨在利用简单重复序列（SSR）标记，研究 41 个橄榄基因型之间的遗传关系，其中包括 11 个著名的土耳其品种和 30 个阿塞拜疆橄榄基因型。在本研究中，19 个 SSR 标记扩增出 115 个多态性 SSR 等位基因。多态性等位基因的数量范围为 3 至 10 个，平均值为 6.05。观察杂合度（Ho）从 0.05 到 0.93，平均值为 0.63，预期杂合度（He）从 0.26 到 0.86，平均值为 0.72。多态信息含量（PIC）范围从 0.23 到 0.85，平均值为 0.68。UPGMA 聚类分析将橄榄基因型分为两个不同的聚类，这两个聚类又分为两个亚群。同样，STRUCTURE 分析将橄榄基因型分为两个不同的基因库（K=2），并通过 STRUCTURE 分析鉴定了四个基因库，代表两个亚群。当 K=4 时，4 个基因库被鉴定出来。橄榄基因型的遗传相似性范围为 0.36 至 0.95。这些结果表明，30 个阿塞拜疆橄榄基因型之间存在高度的遗传变异。来自阿塞拜疆橄榄基因型的“Ayvalık 1”和“Ayvalık 2”与土耳其当地的橄榄品种“ Ayvalık”不同，表明存在同名现象。本研究还表明，阿塞拜疆橄榄基因型与土耳其橄榄品种完全不同，这表明这些橄榄基因型可能是从土耳其以外的其他国家进口到阿塞拜疆的。本研究的结果表明，这些不同的橄榄基因型可能对阿塞拜疆新橄榄品种的发展有用，两国之间的未来育种计划可以通过这些结果得到加强。
