Wong Mei M, Hong Xuechong, Karamariti Eirini, Hu Yanhua, Xu Qingbo
Cardiovascular Division, King's College London BHF Centre.
Cardiovascular Division, King's College London BHF Centre;
J Vis Exp. 2015 Mar 18(97):52565. doi: 10.3791/52565.
The construction of vascular conduits is a fundamental strategy for surgical repair of damaged and injured vessels resulting from cardiovascular diseases. The current protocol presents an efficient and reproducible strategy in which functional tissue engineered vessel grafts can be generated using partially induced pluripotent stem cell (PiPSC) from human fibroblasts. We designed a decellularized vessel scaffold bioreactor, which closely mimics the matrix protein structure and blood flow that exists within a native vessel, for seeding of PiPSC-endothelial cells or smooth muscle cells prior to grafting into mice. This approach was demonstrated to be advantageous because immune-deficient mice engrafted with the PiPSC-derived grafts presented with markedly increased survival rate 3 weeks after surgery. This protocol represents a valuable tool for regenerative medicine, tissue engineering and potentially patient-specific cell-therapy in the near future.
血管导管的构建是外科修复因心血管疾病导致的受损血管的基本策略。当前方案提出了一种高效且可重复的策略，即使用来自人成纤维细胞的部分诱导多能干细胞（PiPSC）生成功能性组织工程血管移植物。我们设计了一种脱细胞血管支架生物反应器，它紧密模拟天然血管内的基质蛋白结构和血流，用于在移植到小鼠体内之前接种PiPSC内皮细胞或平滑肌细胞。这种方法被证明是有利的，因为植入PiPSC衍生移植物的免疫缺陷小鼠在手术后3周存活率显著提高。该方案是再生医学、组织工程以及在不久的将来可能用于患者特异性细胞治疗的宝贵工具。
