Fout G Shay, Cashdollar Jennifer L, Varughese Eunice A, Parshionikar Sandhya U, Grimm Ann C
National Exposure Research Laboratory, U.S Environmental Protection Agency;
National Exposure Research Laboratory, U.S Environmental Protection Agency.
J Vis Exp. 2015 Mar 28(97):52067. doi: 10.3791/52067.
EPA Method 1615 was developed with a goal of providing a standard method for measuring enteroviruses and noroviruses in environmental and drinking waters. The standardized sampling component of the method concentrates viruses that may be present in water by passage of a minimum specified volume of water through an electropositive cartridge filter. The minimum specified volumes for surface and finished/ground water are 300 L and 1,500 L, respectively. A major method limitation is the tendency for the filters to clog before meeting the sample volume requirement. Studies using two different, but equivalent, cartridge filter options showed that filter clogging was a problem with 10% of the samples with one of the filter types compared to 6% with the other filter type. Clogging tends to increase with turbidity, but cannot be predicted based on turbidity measurements only. From a cost standpoint one of the filter options is preferable over the other, but the water quality and experience with the water system to be sampled should be taken into consideration in making filter selections.
美国环境保护局（EPA）方法1615的制定目标是提供一种用于测量环境水和饮用水中肠道病毒和诺如病毒的标准方法。该方法的标准化采样部分通过使至少规定体积的水通过一个正电滤筒式过滤器来浓缩水中可能存在的病毒。地表水和成品水/地下水的规定最小体积分别为300升和1500升。该方法的一个主要局限性是在达到样品体积要求之前过滤器有堵塞的倾向。使用两种不同但等效的滤筒式过滤器选项的研究表明，对于其中一种过滤器类型，10%的样品存在过滤器堵塞问题，而另一种过滤器类型的这一比例为6%。堵塞倾向往往随浊度增加，但不能仅基于浊度测量来预测。从成本角度来看，其中一种过滤器选项比另一种更可取，但在选择过滤器时应考虑水质以及待采样水系统的情况。
