Department of Pulp Biology and Endodontics, Kanagawa Dental University, 82. Inaoka-Cho, Yokosuka, Kanagawa, 238-8580, Japan.
Ministry of Defense Japan Self-Defense Forces Hospital Yokosuka, 1766-1 Tauraminato-Cho, Yokosuka, Kanagawa, 237-0071, Japan.
Odontology. 2024 Jan;112(1):177-184. doi: 10.1007/s10266-023-00827-8. Epub 2023 Jul 11.
Detection of the oral bacterium Fusobacterium nucleatum in colorectal cancer tissues suggests that periodontitis may alter gut microbiota. The purpose of this study was to analyze the influence and infection route of periodontal inflammation caused by F. nucleatum, and microbiota of the gut and surrounding organs (heart, liver, kidney). Wistar female rats were orally inoculated with F. nucleatum to establish an experimental periodontitis model that was confirmed by X-ray imaging and histopathological analysis. The mandibles, gut, liver, heart, and kidneys were collected from the experimental group at 2, 4, and 8 weeks, and from the uninfected control group at 0 weeks, for DNA extraction for PCR amplification and comprehensive microbiota analysis using the Illumina MiSeq platform. Imaging confirmed the onset of periodontitis at 2 weeks post-inoculation, and histopathology showed inflammatory cell infiltration from 2 to 8 weeks. PCR and comprehensive microbiota analysis showed the presence of F. nucleatum in the heart and liver at 2 weeks, and in the liver at 4 and 8 weeks. There were changes of microbiota of the gut, heart, liver, and kidneys at 4 weeks: namely, decreased Verrucomicrobia and Bacteroidetes, and increased Firmicutes. F. nucleatum induced the onset of periodontitis and infected the heart and liver in rats. As the periodontic lesion progressed, the microbiota of the gut, liver, heart, and kidneys were altered.
在结直肠癌组织中检测到口腔细菌福赛斯坦纳菌表明牙周炎可能改变肠道微生物群。本研究旨在分析由福赛斯坦纳菌引起的牙周炎的影响和感染途径，以及肠道和周围器官（心脏、肝脏、肾脏）的微生物群。将雌性 Wistar 大鼠经口接种福赛斯坦纳菌，建立实验性牙周炎模型，通过 X 射线成像和组织病理学分析进行验证。在接种后 2、4 和 8 周，从实验组和未感染的对照组的下颌骨、肠道、肝脏、心脏和肾脏中提取 DNA，用于 PCR 扩增和使用 Illumina MiSeq 平台进行综合微生物群分析。成像证实接种后 2 周出现牙周炎，组织病理学显示从 2 周到 8 周炎症细胞浸润。PCR 和综合微生物群分析显示，在 2 周时心脏和肝脏中存在福赛斯坦纳菌，在 4 和 8 周时肝脏中存在福赛斯坦纳菌。4 周时肠道、心脏、肝脏和肾脏的微生物群发生变化：即厚壁菌门和拟杆菌门减少，而 Firmicutes 增加。福赛斯坦纳菌诱导大鼠牙周炎的发生，并感染心脏和肝脏。随着牙周病损的进展，肠道、肝脏、心脏和肾脏的微生物群发生改变。
