College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China.
College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China.
Int J Biol Macromol. 2015;78:142-8. doi: 10.1016/j.ijbiomac.2015.03.056. Epub 2015 Apr 11.
A water-soluble polysaccharide was isolated from Crassostrea gigas (CGPS-1) by hot-water extraction, isoelectric precipitation, hydrolysis and ultrafiltration without using ethanol. Based on the calibration with Dextran, CGPS-1 had a molecular weight of approximately 6.5×10(6) Da. CGPS-1 was analyzed using high-performance liquid chromatography, Fourier-transform infrared spectroscopy and (1)H and (13)C nuclear magnetic resonance spectroscopy. Based on the data, CGPS-1 was found to be a uniform glucose polymer. The protective effect of CGPS-1 on acute carbon tetrachloride- and chronic ethanol-induced liver injury was investigated in mice. Treatment with CGPS-1 decreased serum aspartate aminotransferase (AST), serum alanine aminotransferase (ALT), and malondialdehyde (MDA) levels; increased superoxide dismutase (SOD) activity; and improved hepatic injury in both the acute and chronic models of liver injury in mice. These results suggest that CGPS-1 possesses potent hepatoprotective activity.
从太平洋牡蛎（Crassostrea gigas）中通过热水提取、等电沉淀、水解和超滤等方法分离出一种水溶性多糖（CGPS-1），无需使用乙醇。基于葡聚糖的标定，CGPS-1 的分子量约为 6.5×10(6) Da。使用高效液相色谱、傅里叶变换红外光谱和（1）H 和（13）C 核磁共振光谱对 CGPS-1 进行了分析。根据这些数据，CGPS-1 被发现是一种均匀的葡萄糖聚合物。在小鼠中研究了 CGPS-1 对急性四氯化碳和慢性乙醇诱导的肝损伤的保护作用。CGPS-1 处理可降低血清天冬氨酸转氨酶（AST）、血清丙氨酸转氨酶（ALT）和丙二醛（MDA）水平；增加超氧化物歧化酶（SOD）活性；并改善急性和慢性肝损伤模型中小鼠的肝损伤。这些结果表明 CGPS-1 具有很强的保肝活性。
